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14-925M Wee1 Protein, active, 250 µg

Active, N-terminal GST-tagged & C-terminal 6His tagged, recombinant, human Wee1, amino acids 214-end. For use in Kinase Assays.

Wee1 Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-925M
250 µg  Also available in 10μg size as catalog # 14-925 and in bulk as catalog # 14-925-K.
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Catalogue Number14-925M
Trade Name
  • Upstate
DescriptionWee1 Protein, active, 250 µg
OverviewActs as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
References
Product Information
Presentation50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.
Quality LevelMQ300
Applications
ApplicationActive, N-terminal GST-tagged & C-terminal 6His tagged, recombinant, human Wee1, amino acids 214-end. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceN-terminal GST-tagged and C-terminal 6His tagged, recombinant, human Wee1 amino acids 214-end expressed by baculovirus in Sf21 insect cells.
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Gene Symbol
  • WEE1
Protein TargetWEE1
Purification MethodPurified using glutathione agarose.
Target Sub-FamilyCK1
UniProt Number
Molecular Weight77 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of peptide LSNLYHQGKFLQTFCGSPLYRRR.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10μg size as catalog # 14-925 and in bulk as catalog # 14-925-K.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
14-925M 04053252832055

Kapcsolódó termékek és alkalmazások

Alternative Packsize

Katalógusszám Leírás
14-925 Wee1 Protein, active, 10 µg

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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Kategóriák

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases