Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

681647 WRN Helicase Inhibitor, NSC 19630 - CAS 72835-26-8 - Calbiochem

The WRN Helicase Inhibitor, NSC 19630, also referenced under CAS 72835-26-8, controls the biological activity of WRN Helicase. This small molecule/inhibitor is primarily used for Cell Signaling applic

 More>>
WRN Helicase Inhibitor, NSC 19630 - CAS 72835-26-8 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: p53 Activator VIII, 1-((1-Oxopropoxy)methyl)-maleimide, 1-((1-Oxopropoxy)methyl)-1H-pyrrole-2,5-dione, MIRA-1

681647
Purchase on Sigma-Aldrich

Áttekintés

Kulcsspecifikációk táblázata

CAS #Empirical Formula
72835-26-8C₈H₉NO₄

Products

KatalógusszámCsomagolás Menny./csomag
681647-5MG Üvegpalack 5 mg
Hozzáadás a Kedvencekhez
Description
OverviewA cell-permeable maleimide compound selectively inhibits Werner syndrome WRN helicase activity (IC50 = 20 µM) over its ATPase and exonuclease activities (19% and <10% inhibition, respectively, at 50 µM), while exhibiting no effect against the helicase activity of two other human RecQ family DNA helicases (RECQ1 and Werner syndrome BLM), Fanconi anemia group J helicase FANCJ, as well as three other E. coli helicases, RecQ, UvrD, and DnaB. The WRN-dependent antiproliferation activity of MIRA-1/NSC 19630 in HeLa 1.2.11 cultures (0% vs. 95% inhibition with or without siRNA-mediated WRN-depletion, respectively, after 48 h 3 µM treatment) is shown to be a direct result of apoptosis induction due to replication forks blockage (20-fold increase in PCNA foci staining after 72 h 2 µM treatment), enhanced DSB (17-fold increase in γ-H2AX foci staining after 72 h 2 µM treatment), and increased S-phase population (from 24% to 42% after 72 h 2 µM treatment). In some cells, MIRA-1/NSC 19630 appears to also exert its apoptotic effect, at least in part, by restoring DNA-binding and transactivation function of some, but not all, p53 mutants.
Catalogue Number681647
Brand Family Calbiochem®
Synonymsp53 Activator VIII, 1-((1-Oxopropoxy)methyl)-maleimide, 1-((1-Oxopropoxy)methyl)-1H-pyrrole-2,5-dione, MIRA-1
References
ReferencesAggarwal, M., et al. 2011. Proc. Natl. Acad. Sci USA 108, 1525.
Bykov, V.J.N., et al. 2005. J. Biol. Chem. 280, 30384.
Product Information
CAS number72835-26-8
FormWhite solid
Hill FormulaC₈H₉NO₄
Chemical formulaC₈H₉NO₄
Structure formula ImageStructure formula Image
Quality LevelMQ100
Biological Information
Purity≥97% by NMR
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Global Trade Item Number
Katalógusszám GTIN
681647-5MG 04055977260205