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681105 Vitronectin, Human Plasma

Vitronectin, Human Plasma MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Serum Spreading Factor, S-Protein

681105
  
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Áttekintés

Replacement Information
Description
OverviewNative vitronectin purified from human plasma. Extracellular matrix protein that promotes cell adhesion. Exists as a mixture of 75 kDa and 65 kDa polypeptides. Biochemically and immunologically distinct from fibronectin. Binds to cells through the RGD sequence in its cell-binding domain.
Intact protein and SDS-PAGE bands react with monoclonal antibodies to vitronectin, bind to heparin, and promote cell adhesion.
M.W. 65,000 and 75,000.
Catalogue Number681105
Brand Family Calbiochem®
SynonymsSerum Spreading Factor, S-Protein
References
ReferencesHayman, E.G., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 4003.
Product Information
CAS number83380-82-9
FormLyophilized
Applications
Biological Information
Purity≥95% by SDS-PAGE
SourcePrepared from plasma of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
681105 0

Documentation

Vitronectin, Human Plasma MSDS

Title

Safety Data Sheet (SDS) 

Vitronectin, Human Plasma Certificates of Analysis

TitleLot Number
681105

References

Hivatkozások áttekintése
Hayman, E.G., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 4003.

Citations

Titulus
  • Laura Belvisi, et al. (2005) Biological and Molecular Properties of a New αvβ3/αvβ5 Integrin Antagonist. Molecular Cancer Therapeutics 4, 1670-1680.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision19-May-2008 RFH
    SynonymsSerum Spreading Factor, S-Protein
    DescriptionNative vitronectin purified from human plasma. Extracellular matrix protein that promotes cell adhesion. Exists as a mixture of ~75 kDa and ~65 kDa polypeptides. Biochemically and immunologically distinct from fibronectin. Intact protein and SDS-PAGE bands react with monoclonal antibodies to vitronectin, bind to heparin, and promote cell adhesion.
    FormLyophilized
    Recommended reaction conditions
    Coating Tissue Culture Dishes with Vitronectin Although the optimal concentration for cell attachment and culture may differ for various cell types and for individual cell culture systems, typically concentrations as low as 50 ng/cm2 promote cell attachment and growth. Coating Procedure 1. Dissolve the desired amount of vitronectin in serum-free medium or PBS. The volume should be sufficient to coat the desired surface evenly (e.g. 1 ml for a 35 mm dish, 3 ml for a 60 mm dish). 2. Add the appropriate amount of distilled material to the culture surface. 3. Incubate at room temperature for 1-2 h. 4. Aspirate the remaining media. 5. Rinse the plates carefully. Avoid scratching the bottom surface of the plates. Store in the refrigerator (4°C) damp or air dried under sterile conditions. This protocol is meant to serve only as a guideline and may need to be modified for specific applications.
    SourcePrepared from plasma of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
    CAS number83380-82-9
    Purity≥95% by SDS-PAGE
    SolubilityDistilled H₂O or aqueous buffers. Equilibrate vial to room temperature prior to reconstitution. Swirl gently to dissolve.
    Storage -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
    Toxicity Standard Handling
    Merck USA index14, 10026
    ReferencesHayman, E.G., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 4003.
    Citation
  • Laura Belvisi, et al. (2005) Biological and Molecular Properties of a New αvβ3/αvβ5 Integrin Antagonist. Molecular Cancer Therapeutics 4, 1670-1680.
    		•