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650200 Trypsin, Bovine Pancreas, High Purity, Endotoxin-Free

Trypsin, Bovine Pancreas, High Purity, Endotoxin-Free MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
650200
  
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Áttekintés

Replacement Information
Description
Overview

This product has been discontinued.

We are offering Trypsin, Bovine Pancreas (Cat. No. 6502) as a possible alternative. Please read the alternative product documentation carefully and contact technical service if you need additional information.





Native trypsin highly purified from bovine pancreas. Endotoxins removed chromatographically. Serine protease which specifically hydrolyzes peptide bonds at the C-terminus side of arginine and lysine residues. May be used for cell harvesting as well as tissue dissociation when combined with elastase and/or collagenase.
Catalogue Number650200
Brand Family Calbiochem®
References
ReferencesNishino, N., et al. 1974. J. Biochem. (Tokyo) 75, 979.
Brown, W., and Wold, F. 1973. Biochemistry 12, 828.
Schroeder, D., et al. 1968. J. Biol. Chem. 243, 2943.
Product Information
CAS number9002-07-7
Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze BAEE to cause an increase in absorbance of 0.003 units at 253 nm per min at 25°C, pH 7.6.
EC number3.4.21.4
FormLiquid
Formulation5 mg per ml in 0.9% sodium chloride. Sterile-filtered.
Applications
Biological Information
Purity≥90% by HPLC (gel filtration)
Specific Activity≥3000 USP units/mg protein
Physicochemical Information
ContaminantsChymotrypsin: ≤40 NF units/mg protein; endotoxins ≤8 EU/mg
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSYN5075000
Safety Information
R PhraseR: 36/37/38-42

Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation.
S PhraseS: 24-26-36/37

Avoid contact with skin.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing and gloves.
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Irritant
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
650200 0

Documentation

Trypsin, Bovine Pancreas, High Purity, Endotoxin-Free MSDS

Title

Safety Data Sheet (SDS) 

Trypsin, Bovine Pancreas, High Purity, Endotoxin-Free Certificates of Analysis

TitleLot Number
650200

References

Hivatkozások áttekintése
Nishino, N., et al. 1974. J. Biochem. (Tokyo) 75, 979.
Brown, W., and Wold, F. 1973. Biochemistry 12, 828.
Schroeder, D., et al. 1968. J. Biol. Chem. 243, 2943.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-May-2008 RFH
DescriptionNative trypsin highly purified from bovine pancreas. A serine protease that specifically hydrolyzes peptide bonds at the C-terminus side of arginine and lysine residues. May be used for cell harvesting as well as tissue dissociation when combined with elastase and/or collagenase. Endotoxins were removed chromatographically.
FormLiquid
Formulation5 mg per ml in 0.9% sodium chloride. Sterile-filtered.
CAS number9002-07-7
RTECSYN5075000
EC number3.4.21.4
Purity≥90% by HPLC (gel filtration)
ContaminantsChymotrypsin: ≤40 NF units/mg protein; endotoxins ≤8 EU/mg
Specific activity≥3000 USP units/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze BAEE to cause an increase in absorbance of 0.003 units at 253 nm per min at 25°C, pH 7.6.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Irritant
Merck USA index14, 9795
ReferencesNishino, N., et al. 1974. J. Biochem. (Tokyo) 75, 979.
Brown, W., and Wold, F. 1973. Biochemistry 12, 828.
Schroeder, D., et al. 1968. J. Biol. Chem. 243, 2943.