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71537 Tag•off™ High Activity rEK

Increased cleavage rate of fusion proteins

Tag•off™ High Activity rEK MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Tag-Off High Activity Enterokinase

71537
  
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Áttekintés

Replacement Information
Description
OverviewTag•off™ High Activity rEK is a highly purified preparation of the catalytic subunit of recombinant human enterokinase that recognizes the identical cleavage site as the native enzyme, AspAspAspAspLys↓ (Gasparian 2003). The Tag•off High Activity rEK substrate binding site (ArgArgArgLys) differs from the bovine enterokinase site (LysArgArgLys) by a single amino acid, which is believed to account for its higher substrate affinity and the resulting higher specific activity. The preparation has been functionally tested for activity with thioredoxin/human epidermal growth factor and thioredoxin/ human IL-13 fusion proteins (Gasparian). The Tag•off High Activity rEK as a highly purified (99% by Coomassie staining) enzyme solution and includes rEK 1X Dilution/Storage Buffer, 10X Cleavage Buffer, and a Cleavage Control Protein.

This product has been discontinued.
Catalogue Number71537
Brand Family Novagen®
SynonymsTag-Off High Activity Enterokinase
References
ReferencesGasparian, M.E., et al. 2003. Protein Expr. and Purif. 31, 133.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme needed to cleave 50 µg of fusion protein in 16 hours at 23°C in a buffer containing 20 mM Tris-HCl, 50 mM NaCl, and 2 mM CaCl₂, pH 7.4.
•50 UTag•Off™ High-activity rEK
•10 µgCleavage Control Protein
•2 ml1X rEK Dilution/Storage Buffer
•1 ml10X rEK Cleavage Buffer
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Multiple Toxicity Values, refer to MSDS
Storage -20°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
71537 0

Documentation

Tag•off™ High Activity rEK Certificates of Analysis

TitleLot Number
71537

References

Hivatkozások áttekintése
Gasparian, M.E., et al. 2003. Protein Expr. and Purif. 31, 133.

User Protocols

Title
TB458 Tag•off™ High Activity rEK