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616402 TACE Substrate II, Fluorogenic

TACE Substrate II, Fluorogenic MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: TNF-α Convertase Enzyme Substrate, Fluorogenic, ADAM-17 Substrate II, Fluorogenic, MCA-PLAQAV(Dpa)RSSSR-NH₂

616402
  
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Áttekintés

Replacement Information
Description
Overview

This product has been discontinued.





A fluorogenic peptide substrate for TACE (Km = ~ 19 µM). Substrate cleavage occurs at the Ala-Val bond. Useful for screening TACE Inhibitors.
Catalogue Number616402
Brand Family Calbiochem®
SynonymsTNF-α Convertase Enzyme Substrate, Fluorogenic, ADAM-17 Substrate II, Fluorogenic, MCA-PLAQAV(Dpa)RSSSR-NH₂
References
ReferencesPatel, I.R., et al. 1998. J. Immunol. 160, 4570.
Van Dyk, D.E., et al. 1997. Bioorg. Med. Chem. Lett. 7, 1219.
Product Information
FormLiquid
FormulationA 4 mM (7.5 mg/ml) solution in DMSO.
Hill FormulaC₆₉H₁₀₃N₂₃O₂₄
Chemical formulaC₆₉H₁₀₃N₂₃O₂₄
Applications
Biological Information
Purity≥90% by HPLC
Physicochemical Information
Peptide SequenceMCA-Pro-Leu-Ala-Gln-Ala-Val-Dpa-Arg-Ser-Ser-Ser-Arg-NH₂[Dpa = N-3-(2,4-Dinitrophenyl)-L-2,3-diaminopropionyl]
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
616402 0

Documentation

TACE Substrate II, Fluorogenic Certificates of Analysis

TitleLot Number
616402

References

Hivatkozások áttekintése
Patel, I.R., et al. 1998. J. Immunol. 160, 4570.
Van Dyk, D.E., et al. 1997. Bioorg. Med. Chem. Lett. 7, 1219.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision13-September-2008 RFH
SynonymsTNF-α Convertase Enzyme Substrate, Fluorogenic, ADAM-17 Substrate II, Fluorogenic, MCA-PLAQAV(Dpa)RSSSR-NH₂
DescriptionA fluorescence energy transfer (FRET) peptide substrate for tumor necrosis factor (TNF)-α convertase (TACE) (Km ~19 µM). Cleavage of this TACE substrate II occurs between the alanine and valine residues. Useful for the screening of TACE inhibitors. Excitation max: ~320 nm; emission max: ~395 nm.
FormLiquid
FormulationA 4 mM (7.5 mg/ml) solution in DMSO.
Recommended reaction conditions50 mM Tricine buffer, pH 7.5, containing 100 mM NaCl, 10 mM CaCl2, and 1 mM ZnCl2, 10 µM TACE Substrate II, 600 nM TACE; incubate at 27°C with mechanical shaking; reaction can be quenched with 50 mM EDTA (final concentration); cleavage monitored at excitation at ~320 nm and emission at ~395 nm.
Chemical formulaC₆₉H₁₀₃N₂₃O₂₄
Peptide SequenceMCA-Pro-Leu-Ala-Gln-Ala-Val-Dpa-Arg-Ser-Ser-Ser-Arg-NH₂[Dpa = N-3-(2,4-Dinitrophenyl)-L-2,3-diaminopropionyl]
Purity≥90% by HPLC
Storage Protect from light
Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesPatel, I.R., et al. 1998. J. Immunol. 160, 4570.
Van Dyk, D.E., et al. 1997. Bioorg. Med. Chem. Lett. 7, 1219.