Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

69839 T4 DNA Ligase - Novagen

69839
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information

Products

KatalógusszámCsomagolás Menny./csomag
69839-3 Üvegpalack 100 u
Description
OverviewT4 DNA Ligase catalyzes the formation of phosphodiester bonds between 3′-hydroxyl and 5′-phosphate groups of adjacent DNA and RNA nucleotides in blunt-end or cohesive-end configurations. The enzyme joins RNA and DNA strands only in duplex molecules and does not join single-stranded nucleic acids. Novagen T4 DNA Ligase is rigorously tested in a blue-white cloning assay under conditions that maximize exposure of DNA ends to any contaminating nucleases. The enzyme is qualified for any ligation or cloning application.
Catalogue Number69839
Brand Family Novagen®
References
Product Information
Unit of Definition4 Weiss units/µl; one Weiss unit is defined as the amount of enzyme required to catalyze the exchange of 1 nmole of ³²P from pyrophosphate into ATP as Norit®-absorbable material in 20 minutes at 37°C. 0.01 Weiss unit is the amount of enzyme required to ligate > 95% of 1 µg bacteriophage λ <i>Hin</i>d III fragments at 16°C in 20 minutes.
Quality LevelMQ200
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
69839-3 04055977258820