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03-0182-00 SMC™ Human IL-12p70 High Sensitivity Immunoassay Kit

The sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human IL-12p70 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-12p70 in human serum and plasma.

SMC™ Human IL-12p70 High Sensitivity Immunoassay Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Single Molecule Counting (SMC®) Research Category: Inflammation & Immunology Research Sub-Category: Inflammatory Disease, Immune Response, Autoimmune disease
03-0182-00
96 well assay  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Species ReactivityKey Applications
R, M, Mk, Ca, FeSingle Molecule Counting (SMC®)
Description
Catalogue Number03-0182-00
Trade Name
  • SMC®
DescriptionSMC™ Human IL-12p70 High Sensitivity Immunoassay Kit
OverviewThe SMC™ Human IL-12p70 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology.
Background InformationInterleukin 12p70 (IL-12p70), belongs to the IL-12 family of cytokine responsible for immune response through the differentiation of T cells to Th1 cells, the induction of IFN gamma production by T and NK cells which block the formation of new blood vessels and the signaling pathways mediated by these cytokines play a role in inflammation, autoimmune disease and infection. IL-12p70 is the heterodimeric 70kDa cytokine made up on subunits p35 and p40.
References
Product Information
Components
  • Assay Buffer
  • IL-12p70 Coated Beads
  • Standard Diluent
  • IL-12p70 Detection Antibody
  • IL-12p70 Standard
  • 10X Wash Buffer
  • Buffer D
  • Elution Buffer B
Precision, %
  • Intra-assay CV% average (n=20): <15%
  • Inter-assay CV% average (n=5): <20%
Applications
ApplicationThe sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human IL-12p70 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-12p70 in human serum and plasma.
Key Applications
  • Single Molecule Counting (SMC®)
Application NotesThis new streamline kit has been improved with the following features over the legacy IL-12 kit, #03-0057-00:
• Improved options for sample prep and incubation
• Lyophilization of the standard provides the kit at one storage condition
• Removal of a plate transfer step
• Increased reagent volumes to accommodate automation
• Validated to run on both SMCxPRO™ and Erenna®
• Matched to WHO standard
Biological Information
Species Reactivity
  • Rat
  • Mouse
  • Monkey
  • Canine
  • Feline
Species Reactivity NoteAlthough IL-12p70 was detectable in rat, mouse, monkey, canine, and feline plasma, the assay has not been validated for these species.
Cross ReactivityCross-reactivity to interferons of other types was tested using SMC™ Human IL-12p70 High Sensitivity Immunoasay Kit with the following results:
rhIL-12p40 was found to be < 2.0% cross-reactive
rhIL-23 was found to be < 1.5% cross-reactive
rhILp35 was not found to be cross-reactive
Accuracy
  • Minimum of 5 serum samples spiked with average recovery of 103% with a range of 100-106%
  • Minimum of 5 plasma samples spiked with average recovery of 97% with a range of 84-102%
Physicochemical Information
Sensitivity
  • Lower Limit of Quantification: 0.1 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96 well assay
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
03-0182-00 04061841064268