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03-0176-00 SMC™ Human IL-10 High Sensitivity Immunoassay Kit

The sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human IL-10 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-10 in human serum and plasma.

SMC™ Human IL-10 High Sensitivity Immunoassay Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Single Molecule Counting (SMC®) Research Category: Inflammation & Immunology Research Sub-Category: Inflammatory Disease, Immune Response, Autoimmune disease
03-0176-00
96 well assay  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Species ReactivityKey Applications
NhP, M, R, Ca, FeSingle Molecule Counting (SMC®)
Description
Catalogue Number03-0176-00
Trade Name
  • SMC®
DescriptionSMC™ Human IL-10 High Sensitivity Immunoassay Kit
OverviewThe SMC™ Human IL-10 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology.
Background InformationInterleukin 10 (IL-10) is an anti-inflammatory cytokine derived from T cells, mononuclear phagocytes and keratinocytes with effects on immunoregulation and inflammation. It has shown to inhibit the synthesis of pro-inflammatory cytokines while also suppressing the antigen-presentation capacity of antigen presenting cells. As well, IL-10 stimulates T cells and mast cells as well as B cell maturation and antibody production.
References
Product Information
Components
  • Assay Buffer
  • IL-10 Coated Beads
  • Standard Diluent
  • IL-10 Detection Antibody
  • IL-10 Standard
  • 10X Wash Buffer
  • Buffer D
  • Elution Buffer B
Precision, %
  • Intra-assay CV% average (n=20): <15%
  • Inter-assay CV% average (n=5): <20%
Applications
ApplicationThe sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human IL-10 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-10 in human serum and plasma.
Key Applications
  • Single Molecule Counting (SMC®)
Biological Information
Species Reactivity
  • Non-human Primate
  • Mouse
  • Rat
  • Canine
  • Feline
Species Reactivity NoteAlthough IL-10 was detectable in non-human primate, mouse, rat, canine, and feline plasma, the assay has not been validated for these species.
Accuracy
  • Minimum of 5 serum samples spiked with average recovery of 87% with a range of 84-93%
  • Minimum of 5 plasma samples spiked with average recovery of 90% with a range of 82-97%
Physicochemical Information
Sensitivity
  • LLOQ range: 0.1-0.2 pg/mL, refer to CoA for lot-specific LLOQ
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96 well assay
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
03-0176-00 04054839753381