Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

SCC458 SCp2 Mouse Mammary Epithelial Cell Line

SCp2 cell line is useful for the study of normal and abnormal mammary epithelial cell phenotypes

ÚjSCC458
≥1X10⁶ cells/vial   
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Catalogue NumberSCC458
DescriptionSCp2 Mouse Mammary Epithelial Cell Line
Background InformationMammary epithelial cell differentiation and reorganization occurs throughout development and in the adult organ during pregnancy and menopause. During these periods in the adult life there is an increased risk for breast cancer, which is the most frequent cancer in women. Breast cancer can be curable when detected at early stages, but it is considered incurable in advance stages with metastasis. The extracellular matrix (ECM) in mammary tissue influences growth and differentiation during development, differentiation, and carcinogenesis (1). Epithelial cells sense ECM modifications through transmembrane receptors like integrins that can influence the expression of malignant phenotypes like increased proliferation and loss of contact inhibition growth (1).

The SCp2 cell line was isolated from the heterogenous CID9 cells by limiting dilution cloning (2). CID9 cells were isolated from a midpregnancy mouse mammary gland. SCp2 cells require exogenously added ECM and lactogenic hormones to express b-casein, under these conditions the cells arrest growth, aggregate, and form alveolar structures that express high levels of milk proteins (2). SCp2 cells are useful to study normal and abnormal mammary epithelial cell phenotypes because their growth and differentiation can be controlled in culture. Activated mutants of M-Ras have been introduced in normal SCp2 cells to study transformation, invasion, and tumor formation in vivo (3).

Source
SCp2 is a murine mammary epithelial cell line that originated from limiting dilution cloning of heterogenous CID9 cells.

References
1. Int J Biochem Cell Biol 2007, 39(11): 1987-1994.
2. Proc Natl Acad Sci USA 1994, 91(26): 12378-12382.
3. Oncogene 2004, 23(6): 1187-1196.
References
Product Information
Quality LevelMQ100
Applications
ApplicationSCp2 cell line is useful for the study of normal and abnormal mammary epithelial cell phenotypes
Key Applications
  • Cell Culture
Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
Biological Information
Cell Line Type
  • Epithelial Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEach vial contains ≥1X10⁶ viable cells.

Cells are tested negative for infectious diseases by a Mouse Essential CLEAR Panel by Charles River Animal Diagnostic Services.

Cells are verified to be of mouse origin and negative for inter-species contamination from rat, human, Chinese hamster, Golden Syrian hamster, and Non-human Primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services

Cells are negative for mycoplasma contamination.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsCells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Packaging Information
Material Size≥1X10⁶ cells/vial
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
SCC458 04065267034638