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HTS057RTA Ready-to-Assay™ PRP Prolactin-Releasing Peptide Family Receptor Frozen Cells

Human PRP / PrRP / GPR10 GPCR frozen cells for Calcium Flux FLIPR Assays.

Host Cell: Chem-1 Entrez Gene Number: NM_004248
HTS057RTA
1 kit  
Purchase on Sigma-Aldrich

Áttekintés

Kulcsspecifikációk táblázata

SpeciesHost CellsProtein Target
Human Chem-1PRP / PrRP / GPR10
Description
Catalogue NumberHTS057RTA
Trade Name
  • Ready-to-Assay
DescriptionReady-to-Assay™ PRP Prolactin-Releasing Peptide Family Receptor Frozen Cells
Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

PRP, encoded by the GPR10 (or hGR3) gene, is a Gq-coupled receptor for prolactin-releasing peptide that is expressed in the pituitary (Hinuma et al., 1998). Genetic studies in rodents indicate that lack of GPR10 leads to hyperphagia, obesity and dyslipidemia (Gu et al., 2004; Watanabe et al., 2005). In humans, genetic variations in GPR10 are associated with lowered blood pressure (Bhattacharyya et al., 2003). Millipore’s cloned human PRP-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant PRP expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at PRP.
Product Information
Components
  • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
  • Fifty (50) mL of Media Component.
Detection methodFluorescent
Quality LevelMQ300
Applications
ApplicationHuman PRP / PrRP / GPR10 GPCR frozen cells for Calcium Flux FLIPR Assays.
Key Applications
  • Calcium Flux Assays
Biological Information
Entrez Gene Number
GPCR ClassA
Host CellsChem-1
Protein TargetPRP / PrRP / GPR10
Target Sub-FamilyProlactin-releasing peptide
Cell Line Type
  • GPCR Cell Lines
Product Usage Statements
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Packaging Information
Material Size1 kit
Global Trade Item Number
Katalógusszám GTIN
HTS057RTA 04053252277207