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HTS095RTA Ready-to-Assay™ Kappa Opioid Receptor Frozen Cells

Ready-to-Assay™ Kappa Opioid Receptor Frozen Cells MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Host Cell: Chem-1 Entrez Gene Number: NM_000912
HTS095RTA
1 kit  
Purchase on Sigma-Aldrich

Különleges ajánlatok

Áttekintés

Replacement Information

Különleges ajánlatok

This product requires a User Agreement (Label License). Please see product datasheet for further information

Kulcsspecifikációk táblázata

SpeciesHost CellsProtein Target
Human Chem-1Kappa / OP2 / KOP / KOR
Description
Catalogue NumberHTS095RTA
Trade Name
  • Ready-to-Assay
DescriptionReady-to-Assay™ Kappa Opioid Receptor Frozen Cells
Background InformationEMD Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. EMD Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

Opiates derived from the opium poppy, Papaver somniferum, have been used for millenia for their anti-diarrheal, analgesic and euphoric properties. More recently, endogenous peptides, enkephalins, dynorphins, and endorphins, were found to bind to the same sites as opiate alkaloids. The receptors for the classical opioids are three related GPCRs, μ, κ, and d, that activate Gi/o to reduce intracellular cAMP levels. Most clinically used opioids function by activation of the μ opioid receptor. Activation of the κ opioid receptor by selective agonists also produces analgesia, primarily mediated by spinal sites, but causes dysphoria and psychosis instead of euphoria. The κ receptor at central and peripheral sites is also largely responsible for the anti-diarrheal effects of opiates (Dhawan et al., 1996). EMD Millipore’s cloned human κ-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant κ expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway.
References
Product Information
Components
  • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
  • Fifty (50) mL of Media Component.
Detection methodFluorescent
Quality LevelMQ100
Applications
Key Applications
  • Calcium Flux Assays
Biological Information
Entrez Gene Number
GPCR ClassA
Host CellsChem-1
Protein TargetKappa / OP2 / KOP / KOR
Target Sub-FamilyOpioid
Cell Line Type
  • GPCR Cell Lines
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
  • Limited to a single use and shall not be propagated and/or re-frozen by licensee.
Storage and Shipping Information
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
HTS095RTA 04053252945502

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ChemiSCREEN™ GPCR Stable Cell Lines, Membranes Preparation and Ready-to-Assay™ Frozen Cells

Millipore offers a large selection of robust and reliable G-protein coupled receptor products, including Stable Cell Lines, Membrane Preparations, and Frozen Cells. See below for GPCR research tools.
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Kapcsolódó termékek gyártója: Brand Facete

Ready-to-Assay™

Kategóriák

Life Science Research > Cell Culture and Systems > Cell Lines > GPCR Frozen Cells
Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Frozen Cells