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			96-Well Plate

Mennyiség	Katalógusszám	A rendelés leírása	Menny./csomag	Listaár
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

03-111 Sigma-AldrichRIPAb+ AUF1 - RIP Validated Antibody and Primer Set

This RIPAb+ AUF1 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

RIPAb+ AUF1 - RIP Validated Antibody and Primer Set MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References

Key Applications: RNA Binding Protein Immunoprecipitation (RIP), Immunoprecipitation, Western Blotting Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins, RNA Binding Protein (RBP)

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Species Reactivity	Key Applications
H, M, R	RIP, IP, WB

Description
Catalogue Number	03-111
Brand Family	Upstate
Trade Name	RIPAb+ Upstate
Description	RIPAb+ AUF1 - RIP Validated Antibody and Primer Set
Overview	RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.
Alternate Names	Heterogeneous nuclear ribonucleoprotein D0 AU-rich element RNA-binding protein 1
Background Information	A+U Rich RNA Binding Factor (AUF1) is comprised of four isoforms of 37, 40, 42, and 45 kDa. Although the role of each isoform has yet to be fully characterized, a direct correlation has been observed between each AUF1 isoform’s binding affinity and its RNA destabilizing activity toward different AREs (A + U rich element in the 3’ untranslated region), with the isoforms p37 and p42 being the most effective. AUF1 is a bcl2 mRNA binding protein and potentially all its isoforms are able to form complexes with the bcl2 ARE. ARE mediated bcl2 mRNA downregulation during apoptosis involves AUF1 and suggest different roles for its four isoforms.

References

Product Information
Format	Purified
Control	Includes negative control rabbit IgG antibody and control primers specific for human FOS.
Presentation	Anti-AUF1 (Rabbit Polyclonal). One vial containing 50 μg of protein A purified rabbit IgG in 50 μL of 70% storage buffer (0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide) and 30% glycerol. Store at -20°C. Normal Rabbit IgG. One vial containing 125 μg Rabbit IgG in 125 μL storage buffer containing 0.05% sodium azide. Store at -20°C. RIP Primers, FOS. One vial containing 75 μL of 5 μM of each primer specific for human FOS. Store at -20°C. FOR: GAG AGC TGG TAG TTA GTA GCA TGT TGA REV: AAT TCC AAT AAT GAA CCC AAT AGA TTA GTT A
Quality Level	MQ100

Applications
Application	This RIPAb+ AUF1 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
Key Applications	RNA Binding Protein Immunoprecipitation (RIP) Immunoprecipitation Western Blotting
Application Notes	Immunoprecipitation from RIP lysate: Representative lot data. RIP lysate from HeLa cells (2 X 10⁶ cell equivalents per IP) was subjected to immunoprecipitation using 0.5 µg of either a normal rabbit IgG or Anti-AUF1 antibody. Precipitated proteins (Lane 1: rabbit IgG, Lane 2: anti-AUF1) and HeLa whole cell lysate (Lane 3) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-AUF1 antibody (1.0 µg/mL). Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00231) (Please see figures). Western Blot Analysis: 0.01-1 µg/mL of a previous lot detected AUF1 in RIPA lysates from HeLa nuclear extract and A431 cells.

Biological Information
Immunogen	Human AUF1 purified by Ni²⁺ affinity column.
Host	Rabbit
Specificity	AUF1 isoforms at 37, 40, 42 and 45 kDa.
Species Reactivity	Human Mouse Rat
Species Reactivity Note	Human. Predicted cross-reactivity with mouse and rat based on sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_001003810.1
Entrez Gene Summary	This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants.
Gene Symbol	HNRNPD AUF1 HNRPD hnRNP D0
UniProt Number	Q14103
UniProt Summary	Function: Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. Binding of RRM1 to DNA inhibits the formation of DNA quadruplex structure which may play a role in telomere elongation. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Subunit structure: Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Part of a complex associated with the FOS mCRD domain and consisting of PABPC1, PAIP1, CSDE1/UNR and SYNCRIP. Interacts with IGF2BP2. Subcellular location: Nucleus. Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Post-translational modification: Arg-345 is dimethylated, probably to asymmetric dimethylarginine. Sequence similarities: Contains 2 RRM (RNA recognition motif) domains.
Molecular Weight	37, 40, 42 and 45 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	RNA Binding Protein Immunoprecipitation: RIP Lysate prepared from HeLa cells (2 X 10⁷ cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal rabbit IgG or 5 µg of Anti-AUF1 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700). Successful immunoprecipitation of AUF1-associated RNA was verified by qPCR using RIP Primers FOS (Please see figures). Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

RNA Binding Protein Immunoprecipitation:
RIP Lysate prepared from HeLa cells (2 X 10⁷ cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal rabbit IgG or 5 µg of Anti-AUF1 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
Successful immunoprecipitation of AUF1-associated RNA was verified by qPCR using RIP Primers FOS (Please see figures).
Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Packaging Information
Material Size	10 assays
Material Package	10 assays per set. Recommended use: ~5 μg of antibody per RIP (dependent upon biological context).

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Katalógusszám	GTIN
03-111	04053252679216

Documentation

RIPAb+ AUF1 - RIP Validated Antibody and Primer Set MSDS

Title
Safety Data Sheet (SDS)

RIPAb+ AUF1 - RIP Validated Antibody and Primer Set Certificates of Analysis

Title	Lot Number
RIPAb+ AUF1 - 2381746	2381746
RIPAb+ AUF1 - 2042805	2042805
RIPAb+ AUF1 - 2492792	2492792
RIPAb+ AUF1 - 3707482	3707482
RIPAb+ AUF1 - 3861954	3861954
RIPAb+ AUF1 - 4003874	4003874
RIPAb+ AUF1 - NG1827444	NG1827444
RIPAb+ AUF1 - NRG1726597	NRG1726597
RIPAb+ AUF1 -2573542	2573542
RIPAb+ AUF1 -2594831	2594831

References

Reference overview	Pub Med ID
Independent exponential feeding of glycerol and methanol for fed-batch culture of recombinant Hansenula polymorpha DL-1. H Moon,S W Kim,J Lee,S K Rhee,E S Choi,H A Kang,I H Kim,S I Hong Applied biochemistry and biotechnology 111 2003 Kivonat megmutatása As a novel feeding strategy for optimizing human epidermal growth factor (hEGF) production with a recombinant Hansenula polymorpha DL-1 using the methanol oxidase (MOX) promoter in H. polymorpha DL-1, independent exponential feeding of two substrates was used. A simple kinetic model considering the cell growth on two substrates was established and used to calculate the respective feeding rates of glycerol and methanol. In the fedbatch culture with methanol-only feeding, the optimal set point of specific growth rate on methanol was found to be 0.10 h-1. When the fed-batch cultures were conducted by the independent feeding of glycerol and methanol, the actual specific growth rate on glycerol and methanol was slightly lower than the set point of specific growth rate. By the uncoupled feeding of glycerol and methanol the volumetric productivity of hEGF increased from 6.4 to 8.0 mg/(L.h), compared with methanol-only feeding.	14645999

Reference overview

Pub Med ID

Kapcsolódó termékek és alkalmazások

Katalógusszám	Leírás
07-260	Anti-AUF1 Antibody

Kapcsolódó termékek gyártója: Brand Facete

Kategóriák

Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Validated Antibodies

Life Science Research > Antibodies and Assays > Primary Antibodies

Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Validated Antibodies

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