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CC300 PureCol®-S, Collagen Standard Solution

Ready-to-use ultrapure collagen type I protein for cell culture applications (20 mL).

CC300
20 mL  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Catalogue NumberCC300
DescriptionPureCol®-S, Collagen Standard Solution
OverviewCollagen is the main structural protein of the extracellular matrix found within various connective tissues of the body. Type I collagen is a major structural component of skin, bone, tendon, and other fibrous connective tissues. In order to provide the most in vivo-like environment for cells, researchers have historically cultured cells inside collagen hydrogels or on top of collagen coated plasticware.
PureCol® is an ultrapure bovine skin derived collagen that is approximately 97% Type I collagen with the remainder being comprised of Type III collagen. It contains a high monomer content as measured by gel permeation chromatography providing product consistency, reproducibility and is cited in over 2000 publications.
PureCol®-S is a soluble atelocollagen in 0.01 N HCI with a pH of 1.9-2.3. PureCol®-S is sterile filtered and is supplied as a ready-to-use solution for cell culture. This product is supplied at 3 mg/mL and can be used as a standard in cell culture applications such as cell adhesion and expansion.

Instructions For Use

Coating Procedure

Note: Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.

1. Remove required quantity of PureCol®-S from the bottle and dispense into a dilution vessel.
2. Dilute PureCol®-S in water to ~50 to 100 µg/ml (~1:30). A 0.01 N HCl solution may also be used.
3. Swirl contents gently until material is completely mixed.
4. Add appropriate amount of diluted PureCol®-S material to the culture surface ensuring that the entire surface is coated.
5. Incubate at room temperature, covered, for 1-2 hours. Aspirate any remaining material. Alternatively, incubate at room temperature until surface is dry.
6. Rinse coated surfaces carefully with sterile medium or PBS, avoid scratching surfaces.
7. Coated surfaces are ready for use. They may also be stored at 2-8°C damp or air dried if sterility is maintained.

3D Gel Preparation Procedure

1. Slowly add 1 part of chilled 10X PBS or 10X culture media to 8 parts of chilled collagen solution with gentle swirling.
2. Adjust pH of mixture to 7.2–7.6 using sterile 0.1 M NaOH. Monitor pH adjustment carefully (pH meter, phenol red, or pH paper).
3. Adjust final volume to a total of 10 parts with sterile water.
4. To prevent gelation, maintain temperature of mixture at 2–10°C.
5. To form gel, warm to 37°C. Allow approximately 90 to 120 minutes for gel formation.

PureCol® is a registered trademark of Advanced BioMatrix, Inc.
Alternate Names
  • Collagen Type I
References
Product Information
Applications
ApplicationReady-to-use ultrapure collagen type I protein for cell culture applications (20 mL).
Key Applications
  • Cell Culture
  • Cell Attachment
Biological Information
Concentration2.9-3.2 mg/mL
HostBovine
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceCollagen Concentration: 2.9-3.2 mg/mL
Collagen Concentration - AAA FIO: 2.9-3.2 mg/mL
Sterility (USP modified): No Growth
Endotoxin LAL: ≤ 1.0 EU/mL
Gel Formation Tube Test: ≤ 40 minutes
Kinetic Gel Test: ≤ 40 minutes
Purity – SDS Page Silver Stain: ≥ 99.9%
pH: 1.9-2.3
Osmolality: ≤ 35 mOsm/Kg H20
Fibrillogenesis: ≥ 0.5 Absorbance Units
Electrophoretic Pattern: Characteristic
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore the PureCol®-S, Collagen Standard Solution at 2-8°C. Aliquot into smaller working aliquots before use to ensure product integrity and performance.
Packaging Information
Material Size20 mL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
CC300 04061841868675