Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

539723 ProteoExtract® Phosphopeptide Enrichment SCIMAC Kit

ProteoExtract® Phosphopeptide Enrichment SCIMAC Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
539723
  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Overview

This product has been discontinued.






The ProteoExtract® Phosphopeptide Enrichment SCIMAC Kit is a useful tool for enrichment of phosphorylated peptides from complex samples by combination of strong cation exchange chromatography (SCX) and immobilized metal ion affinity chromatography (IMAC). The identification of phosphorylation sites is routinely accomplished by mass spectrometry. Captured and eluted phosphopeptide fractions are ready for MALDI- and ESI- mass spectrometry analysis.
Catalogue Number539723
Brand Family Calbiochem®
Application Data
The combination of SCX and IMAC exhibits high selectivity for phosphopeptides by reducing nonspecific binding of peptides from complex mixtures. A complex peptide mixture derived from a tryptic digest of porcine liver extract was spiked with α-casein and two synthetic phosphopeptides. The samples were subsequently processed using (A) an ironoxide-based IMAC material alone or (B) the combination of SCX with zirconium-based IMAC particles as outlined in the Detailed Protocol. Mass spectrometry analysis was performed using ESI-LC/MS equipment operated in positive mode.
Materials Required but Not Delivered• Micropipettes and tips; 10 µl, 200 µl, and 1 ml
• Microcentrifuge and rotor for >10,000 x g
• 1.5 ml microcentrifuge tubes
• Magnetic stand or separator (optional)
References
Product Information
Detection methodMass Spectrometry
Form100 Isolations
FormatPhosphopeptide enrichment
Kit contains10X Phosphobind Buffer, Wash Buffer 1, Wash Buffer 2, Elution Buffer, MagPrep® Phosphobind Particles, SCX Resin, SCX Elute Buffer, and a user protocol.
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Intended useThe ProteoExtract® Phosphopeptide Enrichment SCIMAC Kit is designed to enrich phosphorylated peptides from complex samples using a combination of strong cation exchange chromatography (SCX) and immobilized metal ion affinity chromatography (IMAC) The final phosphopeptide fraction is directly compatible with downstream analysis by MALDI- and ESI mass spectrometry.
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage +2°C to +8°C
Storage ConditionsUpon arrival store the entire contents of the kit at 4°C for long-term storage or at room temperature for up to 6 months.
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Kit contains10X Phosphobind Buffer, Wash Buffer 1, Wash Buffer 2, Elution Buffer, MagPrep® Phosphobind Particles, SCX Resin, SCX Elute Buffer, and a user protocol.
Specifications
Global Trade Item Number
Katalógusszám GTIN
539723 0

Documentation

ProteoExtract® Phosphopeptide Enrichment SCIMAC Kit Certificates of Analysis

TitleLot Number
539723

User Protocols

Title
User Protocol