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577815 PhosphoDetect™ Anti-Tau (pSer³⁹⁶) Rabbit pAb

577815
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Áttekintés

Kulcsspecifikációk táblázata

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody

Products

KatalógusszámCsomagolás Menny./csomag
577815-10T Muanyagampulla 10 t
Description
OverviewRecognizes the ~45-68 kDa Tau protein phosphorylated at Ser396.
Catalogue Number577815
Brand Family Calbiochem®
Application Data
Detection of human tau, phosphorylated at Ser396, by immunoblotting. Samples: Whole cell lysate from control cells with exogenously added human recombinant tau left untreated (lane 1) or treated with GSK-3β (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Tau (pSer396) Rabbit pAb (Cat. No. 577815) (1:1000) pre-incubated without peptide (lanes 1, 2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphoserine containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
References
Referencesdel C. Alonso, A., et al. 2001. J. Biol. Chem. 276, 37967.
Bennecib, M., et al. 2001. FEBS Lett. 490, 15.
Sawamura, N., et al. 2001. J. Biol. Chem. 276, 10314.
Tomidokoro, Y., et al. 2001. Neurosci. Lett. 299, 169.
Bennecib, M., et al. 2000. FEBS Lett. 485, 87.
Hashiguchi, M., et al. 2000. J. Biol. Chem. 275, 25247.
Jenkins, S.M., et al. 2000. Biochem. J. 345, 263.
Maas, T., et al., 2000. J. Biol. Chem. 275, 15733.
Takahashi, M., et al. 2000. Brain. Res. 857, 193.
Tesseur, I., et al. 2000. Am. J. Pathol. 156, 951.
Schneider, A., et al. 1999. Biochemistry 38, 3549.
Sengupta, A., et al. 1998. Arch. Biochem. Biophys. 357, 299.
Wang, J.G., et al. 1998. FEBS Lett. 436, 28.
Kopke, E., et al. 1993. J. Biol. Chem. 268, 24374.
Arigada, P.A., et al. 1992. Neurology 42, 631.
Drubin, D.G. and Kirschner, M.W. 1986. J. Cell. Biol. 103, 2739.
Product Information
FormLiquid
FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.2-7.4.
Positive controlRecombinant human tau treated with GSK-3β
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1:1000)
Application CommentsWhen used at a 1:1000 dilution, there is sufficient antibody for 10 immunoblots at 10 ml/blot. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁹⁶ phosphorylation site of human Tau
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Global Trade Item Number
Katalógusszám GTIN
577815-10T 04055977265927