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PK1010 PhosphoDetect™ Anti-Syk (pTyr525/526) Rabbit pAb

PK1010
Purchase on Sigma-Aldrich

Áttekintés

Kulcsspecifikációk táblázata

Species ReactivityHostAntibody Type
H, MRbPolyclonal Antibody

Products

KatalógusszámCsomagolás Menny./csomag
PK1010-50UL Muanyagampulla 50 ul
Description
OverviewRecognizes the ~72 kDa Syk protein phosphorylated at Tyr525/526 in human Syk or Tyr519/520 in mouse Syk in 293T cells expressing wild-type Syk and CD8.
Catalogue NumberPK1010
Brand Family Calbiochem®
Application Data
Detection of human Syk, phosphorylated on Tyr525/526, by immunoblotting. Samples: Whole cell lysate from 293T cells transfected with wild-type Syk and CD8, left untreated (left lane) or treated with phosphatase (right lane). Primary antibody: PhosphoDetect™ Anti-Syk (pTyr525/526) Rabbit pAb (Cat. No. PK1010) (1:1000). Detection: chemiluminescence.
References
ReferencesCoopman, P.J., et al. 2000. Nature 406, 742.
Turner, M., et al. 2000. Immunol. Today 21, 148.
Zang, J., et al. 2000. J. Biol. Chem. 275, 35442.
Chu, D.H., et. al. 1998. Immunol. Rev. 165, 167.
Cheng, A.M. and Chan, A.C. 1997. Curr. Opin. Immunol. 9, 528.
Kurosaki, T. 1997. Curr. Opin. Immunol. 9, 309.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
Positive control293T cells transfected with wild-type Syk and CD8
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (1:1000)
Immunoblotting (1:1000)
Application CommentsTyr525/526 represent the phosphorylation sites in human Syk, while Tyr519/520 are the equivalent phosphorylation sites in mouse Syk. For immunoblotting the membrane should be incubated with antibody diluted in 5% BSA, 1X TBS, 0.1% Tween®-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr525/526 phosphorylation sites of human Syk
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsDo not aliquot.
Global Trade Item Number
Katalógusszám GTIN
PK1010-50UL 04055977227017