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527460 PhosphoDetect™ Anti-PKR (pThr⁴⁵¹) Rabbit pAb

This PhosphoDetect™ Anti-PKR (pThr⁴⁵¹) Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry for the detection of PKR (pThr⁴⁵¹).

PhosphoDetect™ Anti-PKR (pThr⁴⁵¹) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Double-stranded RNA-dependent Protein Kinase, Phospho-Specific (Thr⁴⁵¹)

527460
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Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Species ReactivityHostAntibody Type
H, MRbPolyclonal Antibody

Products

KatalógusszámCsomagolás Menny./csomag
527460-10T Muanyagampulla 10 t
Description
OverviewRecognizes the ~65-68 kDa PKR protein phosphorylated at Thr451.
Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
Catalogue Number527460
Brand Family Calbiochem®
SynonymsAnti-Double-stranded RNA-dependent Protein Kinase, Phospho-Specific (Thr⁴⁵¹)
Application Data
Detection of human PKR, phosphorylated on Thr 451, by immunoblotting. Samples: Whole cell lysates from HeLa cells left unstimulated (lane 1) or stimulated with IFNγ and calyculin (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-PKR (pThr 451) Rabbit pAb (Cat. No. 527460) (1:000) pre-incubated without peptide (lanes 1-2), non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
References
ReferencesCuddihy, A.R., et al.1999. Oncogene 8, 2690.
Srivastava, S.P., et al. 1998. J. Biol. Chem. 273, 2416.
Clemens, M.J. 1997. Int. J. Biochem. Cell Biol. 29, 945.
Product Information
FormLiquid
FormulationIn Dulbecco's PBS (without Mg+2 or Ca+2), 0.1% BSA, 50% glycerol, pH 7.3.
Positive controlIFNγ-stimulated HeLa cells.
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunohistochemistry
Application NotesImmunoblotting (1:1000)
Immunohistochemistry (see comments)
Application CommentsWhen used at a 1:1000 dilution there is sufficient antibody for 10 blots at 10 ml/blot. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal dilution.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Thr⁴⁵¹ phosphorylation site of human PKR (double-stranded RNA-dependent protein kinase)
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
527460-10T 04055977270396

Documentation

PhosphoDetect™ Anti-PKR (pThr⁴⁵¹) Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

PhosphoDetect™ Anti-PKR (pThr⁴⁵¹) Rabbit pAb Certificates of Analysis

TitleLot Number
527460

References

Hivatkozások áttekintése
Cuddihy, A.R., et al.1999. Oncogene 8, 2690.
Srivastava, S.P., et al. 1998. J. Biol. Chem. 273, 2416.
Clemens, M.J. 1997. Int. J. Biochem. Cell Biol. 29, 945.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision13-August-2007 RFH
SynonymsAnti-Double-stranded RNA-dependent Protein Kinase, Phospho-Specific (Thr⁴⁵¹)
ApplicationImmunoblotting (1:1000)
Immunohistochemistry (see comments)
Application Data
Detection of human PKR, phosphorylated on Thr 451, by immunoblotting. Samples: Whole cell lysates from HeLa cells left unstimulated (lane 1) or stimulated with IFNγ and calyculin (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-PKR (pThr 451) Rabbit pAb (Cat. No. 527460) (1:000) pre-incubated without peptide (lanes 1-2), non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
DescriptionRabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phophorylation site, followed by immunoaffinity chromatography. Recognizes the ~65-68 kDa PKR protein phosphorylated at Thr451.
BackgroundPKR is an interferon-inducible serine/threonine kinase activated by double stranded RNA. The activation of PKR is accompanied by phosphorylation at multiple sites, including Thr446 and Thr451 in the PKR activation loop. PKR phosphorylates the α-subunit of the eukaryotic initiation factor-2 and inhibits protein synthesis. PKR also phosphorylates p53 and plays a role in the interferon response to viral infections.
HostRabbit
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Thr⁴⁵¹ phosphorylation site of human PKR (double-stranded RNA-dependent protein kinase)
IsotypeIgG
Specieshuman, mouse
Positive controlIFNγ-stimulated HeLa cells.
FormLiquid
FormulationIn Dulbecco's PBS (without Mg+2 or Ca+2), 0.1% BSA, 50% glycerol, pH 7.3.
Preservative≤0.1% sodium azide
CommentsWhen used at a 1:1000 dilution there is sufficient antibody for 10 blots at 10 ml/blot. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal dilution.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesCuddihy, A.R., et al.1999. Oncogene 8, 2690.
Srivastava, S.P., et al. 1998. J. Biol. Chem. 273, 2416.
Clemens, M.J. 1997. Int. J. Biochem. Cell Biol. 29, 945.