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DR1027 PhosphoDetect™ Anti-MDM2 (pSer¹⁶⁶) Rabbit pAb

PhosphoDetect™ Anti-MDM2 (pSer¹⁶⁶) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Ant-Murine Double Minute Chromosome-2

DR1027
  
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Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~90 kDa (apparent MW) MDM2 protein phosphorylated at Ser166 in IGF-1-treated MCF-7 cells.
Catalogue NumberDR1027
Brand Family Calbiochem®
SynonymsAnt-Murine Double Minute Chromosome-2
Application Data
Detection of human MDM2, phosphorylated at Ser166, by immunoblotting. Samples: Whole cell lysate from MCF7 cells treated with IGF-1 for the indicated times. Primary antibody: PhosphoDetect™ Anti-MDM2 (pSer166) Rabbit pAb (Cat. No. DR1027) (1:1000). Detection: chemiluminescence.
References
ReferencesMayo, L.D., and Donner, D.B. 2001. Proc. Natl. Acad. Sci. USA 98, 11598.
Zhou, B.P., et al. 2001. Nat. Cell. Biol 3, 973.
Grossman, S.R., et al. 1998. Mol. Cell 2, 405.
Haupt, Y., et al. 1997. Nature 387, 296.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
Positive controlMCF7 cells treated with IGF-1
PreservativeNone
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1:1000)
Application CommentsDoes not recognize unphosphorylated MDM2. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁶⁶ phosphorylation site of human MDM2
ImmunogenHuman
HostRabbit
IsotypeIgG
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsDo not aliquot.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
DR1027 0

Documentation

PhosphoDetect™ Anti-MDM2 (pSer¹⁶⁶) Rabbit pAb Certificates of Analysis

TitleLot Number
DR1027

References

Hivatkozások áttekintése
Mayo, L.D., and Donner, D.B. 2001. Proc. Natl. Acad. Sci. USA 98, 11598.
Zhou, B.P., et al. 2001. Nat. Cell. Biol 3, 973.
Grossman, S.R., et al. 1998. Mol. Cell 2, 405.
Haupt, Y., et al. 1997. Nature 387, 296.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision23-August-2007 RFH
SynonymsAnt-Murine Double Minute Chromosome-2
ApplicationImmunoblotting (1:1000)
Application Data
Detection of human MDM2, phosphorylated at Ser166, by immunoblotting. Samples: Whole cell lysate from MCF7 cells treated with IGF-1 for the indicated times. Primary antibody: PhosphoDetect™ Anti-MDM2 (pSer166) Rabbit pAb (Cat. No. DR1027) (1:1000). Detection: chemiluminescence.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~90 kDa (apparent MW) MDM2 protein phosphorylated at Ser166.
BackgroundMDM2 is an ubiquitin ligase that plays a central role in the regulation of p53. MDM2 is phosphorylated on Ser166 and Ser186 by ATM. This phosphorylation of MDM2 increases its interactions with p300 allowing the assembly of the protein complex necessary for MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, which contributes to an increase in the degradation of p53.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁶⁶ phosphorylation site of human MDM2
IsotypeIgG
Specieshuman, mouse, rat
Positive controlMCF7 cells treated with IGF-1
FormLiquid
FormulationIn 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
PreservativeNone
CommentsDoes not recognize unphosphorylated MDM2. Antibody should be titrated for optimal results in individual systems.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsDo not aliquot.
Toxicity Standard Handling
ReferencesMayo, L.D., and Donner, D.B. 2001. Proc. Natl. Acad. Sci. USA 98, 11598.
Zhou, B.P., et al. 2001. Nat. Cell. Biol 3, 973.
Grossman, S.R., et al. 1998. Mol. Cell 2, 405.
Haupt, Y., et al. 1997. Nature 387, 296.