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L2223 Papain solution (61.25 mg/l)

L2223
  
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Áttekintés

Replacement Information
Description
OverviewPapain is able to hydrolyse proteins and peptides preferably those with basic amino acids. The ready-to-use preparation (61.25 mg/l) in PBS w/o Ca2+, Mg2+ is derived from papaya fruit (Carica papaya). If compared to the usual Trypsin, Papain preparation has a limited shelf life of 36 hrs at +2-8°C. After thawing, Papain should consequently be divided into aliquots and re-frozen.
Specifications:

Sterility: Pass
Functional Test: Pass
pH: 7.1-7.5
Osmolality: 0.270-0.310 Osm/kg H2O
Endotoxin: Pass
Color: Colorless
Appearance: Clear
Additional Information:
Application Papain solution:
• for passage of cell cultures, supernatant is discharged
•cells washed with 5 ml PBS (w/o Ca2+, Mg2+; Cat. No. L 1825/0)
• supernatant is discharged
• supply culture flasks with (depending on size) 1.0 - 2.5 ml Papain solution
• incubate culture for 5 - 7 min (at +15-25°C) while gently moving the flask, and observe separating progress under a microscope suitable for the survey of cells
• transfer cells into a sterile centrifugal tube and centrifuge at 300xg for 10 min
• discharge supernatant and resuspend pellet in 15 ml culture medium
• repeat centrifugation, resuspend pellet in medium prior to seeding it to culture flask
Catalogue NumberL2223
Brand Family Calbiochem®
References
Product Information
Quality LevelMQ300
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Storage -20°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
L2223 0

Documentation