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SCM152 PLTGold® Human Platelet Lysate

Heparin-free Human Platelet Lysate (hPL) for Mesenchymal Stem Cell Culture. 500ml.

PLTGold® Human Platelet Lysate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Culture, Stem Cell Culture
SCM152
500 mL  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Key ApplicationsSpecies
CULT, CultHuman
Description
Catalogue NumberSCM152
DescriptionPLTGold® Human Platelet Lysate
Background InformationPLTGold® Human Platelet Lysate is a Xeno-Free cell culture supplement that is a superior alternative to fetal bovine serum (FBS) for use in human mesenchymal stem cell cultures. PLTGold is an unfractionated product derived from human platelets that does not require the addition of heparin. It remains clot-free with performance equivalent to the original PLTMax® platelet lysate. Optimal growth of MSCs can be achieved with 5% PLTGold. PLTGold been used to effectively to grow mesenchymal stem cells derived from other species including mouse, rabbit and porcine.

PLTGold Human Platelet Lysate is derived from normal human donor platelets collected at U.S. blood centers. Multiple donors units are pooled in large batch sizes and manufactured to produce a consistent product. Human Platelet Lysate is for research use only (RUO) and not intended for human or animal diagnostic or therapeutic use.
References
Product Information
Applications
ApplicationHeparin-free Human Platelet Lysate (hPL) for Mesenchymal Stem Cell Culture. 500ml.
Key Applications
  • Cell Culture
  • Stem Cell Culture
Application NotesInstructions for Use

1.Cell seeding should be performed following the general guidelines for the specific cell type. For Mesenchymal Stem Cells (MSCs), cells are typically plated at approximately 2x10^3 – 5x10^3 cells per cm2.
2. For MSCs, PLTGold can be used at 5% vol/vol in a typical cell culture medium such as DMEM (Sigma D5671) or α-MEM (Sigma M8042). If the basic media doesn’t contain Glutamine, a source of L-Glutamine (Sigma G7513) will need to be added to the media at a final concentration of 2mM. For other types of cells, the concentration of PLTGold will need to be titrated according to the application (a titration from 2% vol/vol to 10% vol/vol is recommended to establish the percentage of PLTGold needed for the cell type to use).
3. Do not allow primary MSC confluence to exceed 70-80%.
Biological Information
Media FormLiquid
Stem Cell Type
  • Mesenchymal Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceAll human derived materials are obtained from government inspected facilities and are of US origin. Each donor unit was approved for human use and has been tested for infectous diseases including HIV-1, HIV-2, HIV-NAT, HCV, HBsAg, and RPR for Syphilis.

Each lot is extensively tested for the following parameters:
Sterility Testing: Negative for growth
pH: 6.8-7.8
Total protein: 4.0-6.5 g/dl
Osmolality: 250-330 mOsm/kg
Endotoxin USP <85>: <10 EU/mL
Mycoplasma: Not detected
Cell Growth: Pass
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -80°C upon receipt. Thaw at room temperature or in a water bath. After thawing, aliquot and store at -20°C or colder. Long term storage at -80°C is recommended. Multiple freeze/thaw cycles are not recommended.
Packaging Information
Material Size500 mL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
SCM152 04054839484094