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14-452-D PDK1

Recombinant human PDK1, full length, with N-terminal GST tag, for use in enzyme kinetics, screening inhibitors, and selectivity profiling.

PDK1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-452-D
10 μg  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Catalogue Number14-452-D
Brand Family Upstate
Trade Name
  • Upstate
DescriptionPDK1
OverviewRecombinant human PDK1, full length, with N-terminal GST tag
References
Product Information
Presentation40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, and 3 mM DTT
Quality LevelMQ300
Applications
ApplicationRecombinant human PDK1, full length, with N-terminal GST tag, for use in enzyme kinetics, screening inhibitors, and selectivity profiling.
Key Applications
  • Kinase Assay
Biological Information
Concentration0.5 mg/ml
Purity≥80%
SourceSf9 insect cells
Specific Activity5.02 pmol/min/µg
Entrez Gene Number
Gene Symbol
  • PDPK1
  • MGC20087
  • PDK1
  • PkB-like
  • PRO0461
  • MGC35290
  • hPDK1
Protein TargetPDK1
Target Sub-FamilyAGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: O15530 # Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
SIZE: 556 amino acids; 63152 Da
SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
TISSUE SPECIFICITY: Appears to be expressed ubiquitously.
PTM: Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
SIMILARITY: SwissProt: O15530 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily. & Contains 1 PH domain. & Contains 1 protein kinase domain.
Molecular Weight89 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Kinase assay: Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 EM ATP, 0.01% Brij-35 and 2 EM substrate (SER/THR 6, Invitrogen) at room temperature for 1 hour.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for > 6 month at –80°C from date of receipt.
Packaging Information
Material Size10 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
14-452-D 04055977276565