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481418 NEMO NOA Peptide, Cell-Permeable, A-UBI - Calbiochem

NEMO NOA Peptide, Cell-Permeable, A-UBI - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: NEMO Ubiquitin Binding Domain Inhibitor

481418
  
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Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Empirical Formula
C₂₁₅H₃₄₇N₇₁O₅₀S₁ plus TFA and water
Description
Overview

This product has been discontinued.



A cell-permeable 37-mer peptide derived from the NEMO NOA ubiquitin binding site (294-314 of mouse sequence with D304R), and fused with an Antennapedia cell penetrating protein (A) at the N-terminus. It is shown to attenuate NF-κB activation via blocking interactions between K63-polyubiquitin chains and the NOA ubiquitin binding domain of NEMO (Kd = 17 µM for CC2-LZ/A-UBI complex). The biotin-labled A-UBI specifically inhibits the LPS-induced NF-κB activation (IC50 = 1.1 µM, compared with 23 µM for IKKβ derived A-NBD) and TNF-α induced NF-κB activation (IC50 = 21 µM) in 70Z/3-C3 pre-B lymphocytes. It displays an inhibitory effect on nuclear translocation of NF-κB p65 and IKK kinase activity at 10 µM, but does not significantly affect other signaling pathways such as MAPK/ERK, MAPK/JNK, NF-κB and NFAT.
Catalogue Number481418
Brand Family Calbiochem®
SynonymsNEMO Ubiquitin Binding Domain Inhibitor
References
ReferencesChiaravalli, J., et al. 2011. Biochem. Pharm. 82, 1163.
Product Information
FormWhite powder
Hill FormulaC₂₁₅H₃₄₇N₇₁O₅₀S₁ plus TFA and water
Chemical formulaC₂₁₅H₃₄₇N₇₁O₅₀S₁ plus TFA and water
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by HPLC
Physicochemical Information
Peptide SequenceRQIKIWFQNRRMKWKKLKAQADIYKARFQAERHAREK
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
481418 0

Documentation

NEMO NOA Peptide, Cell-Permeable, A-UBI - Calbiochem MSDS

Title

Safety Data Sheet (SDS) 

NEMO NOA Peptide, Cell-Permeable, A-UBI - Calbiochem Certificates of Analysis

TitleLot Number
481418

References

Hivatkozások áttekintése
Chiaravalli, J., et al. 2011. Biochem. Pharm. 82, 1163.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-August-2012 JSW
SynonymsNEMO Ubiquitin Binding Domain Inhibitor
DescriptionA cell-permeable 37-mer peptide derived from the NEMO NOA ubiquitin binding site (294-314 of mouse sequence with D304R), and fused with an Antennapedia cell penetrating protein (A) at the N-terminus. It is shown to attenuate NF-κB activation via blocking interactions between K63-polyubiquitin chains and the NOA ubiquitin binding domain of NEMO (Kd = 17 µM for CC2-LZ/A-UBI complex). The biotin-labled A-UBI specifically inhibits the LPS-induced NF-κB activation (IC50 = 1.1 µM, compared with 23 µM for IKKβ derived A-NBD) and TNF-α induced NF-κB activation (IC50 = 21 µM) in 70Z/3-C3 pre-B lymphocytes. It displays an inhibitory effect on nuclear translocation of NF-κB p65 and IKK kinase activity at 10 µM, but does not significantly affect other signaling pathways such as MAPK/ERK, MAPK/JNK, NF-κB and NFAT.
FormWhite powder
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₂₁₅H₃₄₇N₇₁O₅₀S₁ plus TFA and water
Peptide SequenceRQIKIWFQNRRMKWKKLKAQADIYKARFQAERHAREK
Purity≥95% by HPLC
SolubilityH₂O (50 mg/ml)
Storage Protect from light
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesChiaravalli, J., et al. 2011. Biochem. Pharm. 82, 1163.