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71256 MagPrep® Bacterial Genomic DNA Kit

Efficient isolation of genomic DNA from Gram-positive and Gram-negative bacterial species

MagPrep® Bacterial Genomic DNA Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
71256
  
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Áttekintés

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Description
Overview

This product has been discontinued.





The MagPrep® Bacterial Genomic DNA Kit is designed for purification of genomic DNA from both Gram-positive and Gram-negative bacteria. This kit combines the advantages of a silica-based resin with magnetic-based separation. The method is based on the lysis of bacterial cells in proximity to magnetic silica particles, and the immobilization of large DNA molecules on the surface of the particles. The resulting DNA:magnetic particle complex is washed to remove impurities and purified genomic DNA is eluted. The procedure for Gram-negative bacteria can be performed in as little as 30 minutes, and genomic DNA from Gram-positive bacteria can be purified in as little as 75 minutes, with much of the procedure being hands-off time. Yields of up to 30 µg of genomic DNA can be achieved with 1 ml of culture. Genomic DNA prepared with MagPrep Bacterial Genomic DNA Kit is suitable for a variety of downstream applications including restriction endonuclease digestion, RFLP analysis, Southern blotting, library construction, and many PCR-based applications.
Table 1. Strains processed successfully with the
MagPrep Bacterial Genomic DNA Kit
Aerococcus viridans
Agrobacterium radiobacter
Alcaligenes faecalis
Bacillus cereus
Bacillus subtilis
Citrobacter freundii
Enterobacter cloacae
Enterococcus faecalis
Enterococcus faecium
Erysipelothrix rhusiopathiae
Escherichia coli B
Escherichia coli K-12
Klebsiella pneumoniae

Lactobacillus casei
Lactococcus lactis
Leuconostoc mesenteroides
Listeria innocua plus 12 subspecies
Micrococcus luteus
Proteus vulgaris
Salmonella enterica
Serratia marcescens
Shigella sonnei
Staphylococcus aureus
Staphylococcus simulans
Vibrio parahemolyticus
Yersinia enterocolitica

Catalogue Number71256
Brand Family Novagen®
References
Product Information
•2 × 1 mlMagPrep Silica Particles
•10 mlBacterial Resuspension Buffer
•0.1 mlRNAse A Solution
•20 mgLysozyme, Chicken Egg White
•0.5 mlProteinase K Solution
•10 mlMagPrep BG Lysis Buffer
•2 × 75 mlMagPrep BG Wash Buffer
•10 mlMagPrep BG Elute Buffer
DeclarationMagPrep is a registered trademark of EMD Chemicals, Inc.
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage Multiple storage requirements
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
71256 0