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444248 MMP-13, Proenzyme, His•Tag®, Human, Recombinant, S. frugiperda

MMP-13, Proenzyme, His•Tag®, Human, Recombinant, S. frugiperda MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Collagenase-3

444248
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Áttekintés

Products

KatalógusszámCsomagolás Menny./csomag
444248-10UG Muanyagampulla 10 μg
Hozzáadás a Kedvencekhez
Description
OverviewFull-length recombinant, human MMP-13 fused to a His•Tag® sequence and expressed in S. frugiperda insect cells. This 452 amino acid proenzyme contains the N-terminal propeptide, the Ca2+- and Zn2+-binding catalytic domain, the hinge region, and the C-terminal hemopexin domain. Hydrolyzes collagen type II 5 to 6 times faster than collagens Type I and III. Exhibits high activity towards gelatin and degrades α1-antichymotrypsin and plasminogen activator inhibitor-2 (PAI-2).
Note: 1 mU = 1 milliunit.
Catalogue Number444248
Brand Family Calbiochem®
SynonymsCollagenase-3
References
ReferencesHerget, J., et al. 2003. Am. J. Physiol. 285, 4199.
Nagase, H. 1997. Biol. Chem. 378, 151.
Knäuper, V., et al. 1996. J. Biol. Chem. 271, 1544.
Knauper, V., et al. 1996. J. Biol. Chem. 271, 17124.
Pei, D., and Weiss, S.J. 1996. J. Biol. Chem. 271, 9135.
Knight, C.G., et al. 1992. FEBS Lett. 296, 263.
Product Information
Unit of DefinitionOne unit is defined as the amount of APMA-activated enzyme that will hydrolyze 1.0 µmol MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH₂ (<a href = "/Products/ProductDisplay.asp→catNO=03-32-5032">Cat. No. 03-32-5032</a>) per min at 37°C, pH 7.5.
FormLiquid
FormulationIn 150 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, pH 7.5.
Quality LevelMQ100
Biological Information
Purity≥90% by SDS-PAGE
Specific Activity≥50 mU/mg protein
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Global Trade Item Number
Katalógusszám GTIN
444248-10UG 04055977186482