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HSTCSF28SPMX21 MILLIPLEX® Human High Sensitivity T Cell Panel 21-Plex Premix Serum-Free. Available in China only, all other countries please order HSTCMAG28SPMX21

Simultaneous analyze low levels of cytokine and chemokine biomarkers with the High Sensitivity Bead-Based Multiplex Assays using the Luminex technology, in cell culture samples.

HSTCSF28SPMX21
96-well plate  96 well plate
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Catalogue NumberHSTCSF28SPMX21
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® Human High Sensitivity T Cell Panel 21-Plex Premix Serum-Free. Available in China only, all other countries please order HSTCMAG28SPMX21
Background InformationCytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells) and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Consequently, research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process.

Low levels of inflammation are involved in many clinical and sub-clinical disease states, such as autoimmune disease, cardiovascular disease, diabetes, neurological disorders and cancer. Measuring picogram levels of cytokines, therefore, is critical for understanding the pathogenesis of these diseases.
References
Product Information
Detection methodLuminex xMAP
ConfigurationPremixed
Precision, %
  • Inter-assay: <15%
  • Intra-assay: <10%
Multiplex Ordering RequirementsPremixed
Panel TypeMAGNETIC Cytokines/Chemokines
Quality LevelMQ200
Applications
ApplicationSimultaneous analyze low levels of cytokine and chemokine biomarkers with the High Sensitivity Bead-Based Multiplex Assays using the Luminex technology, in cell culture samples.
Key Applications
  • Multiplexing
Application NotesThis kit requires an overnight incubation.
Because there are unique features concerning sample prep and assay set-up, please read the entire protocol before running the assay.
This kit may be used for the analysis of all or any combination of the Human High Sensitivity T Cell Serum-Free panel analytes in tissue/cell lysate and culture supernatant.
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
Analytes Available
  • Fractalkine
  • GM-CSF
  • IFNγ
  • IL-1β
  • IL-2
  • IL-4
  • IL-5
  • IL-6
  • IL-7
  • IL-8
  • IL-10
  • IL-12 (p70)
  • IL-13
  • IL-17A
  • IL-21
  • IL-23
  • ITAC
  • MIP-1α
  • MIP-1β
  • MIP-3α
  • TNF-α
Cross ReactivityThere is no detectable cross-reactivity within the panel.
Accuracy
  • 98% – 107%
Physicochemical Information
Sensitivity
  • 0.11 - 8.17 pg/mL (Overnight Incubation minDC)
  • Please see kit protocol for individual assay sensitivities.
Linearity of DilutionPlease contact technical service for information
Standard Curve Range
  • Fractalkine: 18.3 – 75,000 pg/mL
  • GM-CSF: 1.22 – 5,000 pg/mL
  • IFNγ: 0.61 – 2,500 pg/mL
  • IL-1β: 0.49 – 2,000 pg/mL
  • IL-2: 0.49 – 2,000 pg/mL
  • IL-4: 1.83 – 7,500 pg/mL
  • IL-5: 0.49 – 2,000 pg/mL
  • IL-6: 0.18 – 750 pg/mL
  • IL-7: 0.37 – 1,500 pg/mL
  • IL-8: 0.31 – 1,250 pg/mL
  • IL-10: 1.46 – 6,000 pg/mL
  • IL-12 (p70): 0.49 – 2,000 pg/mL
  • IL-13: 0.24 – 1,000 pg/mL
  • IL-17A: 0.73 – 3,000 pg/mL
  • IL-21: 0.24 – 1,000 pg/mL
  • IL-23: 7.93 – 32,500 pg/mL
  • ITAC: 1.46 – 6,000 pg/mL
  • MIP-1α: 0.31 – 1,250 pg/mL
  • MIP-1β: 0.92 – 3,750 pg/mL
  • MIP-3α: 0.61 – 2,500 pg/mL
  • TNFα: 0.45 – 1,750 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
HSTCSF28SPMX21 04065267238371

Documentation

MILLIPLEX® Human High Sensitivity T Cell Panel 21-Plex Premix Serum-Free. Available in China only, all other countries please order HSTCMAG28SPMX21 MSDS

Title

Safety Data Sheet (SDS) 

User Guides

Title
User Guide-HSTCSF28SK

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