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ASK26 MDM2 Antibody Sampler Kit-Human

MDM2 Antibody Sampler Kit-Human MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Murine Double Minute Chromosome-2

ASK26
  
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Áttekintés

Replacement Information
Description
Overview

This product has been discontinued.





Contains 20 µg of each of the following antibodies: Anti-MDM2 (Cat. Nos. OP144, OP145, and OP146). Please see individual product descriptions for more information.
Catalogue NumberASK26
Brand Family Calbiochem®
SynonymsAnti-Murine Double Minute Chromosome-2
Application Data
References
ReferencesMarchetti, A., et al. 1995. J. Pathol. 175, 31.
Barak, Y., et al. 1993. EMBO J. 12, 461.
Ladanyi, M., et al. 1993. Cancer Res. 53, 16.
Leach, F. S., et al. 1993. Cancer Res. 53, 2231.
Oliner, J. D., et al. 1993. Nature 362, 857.
Momand, J., et al. 1992. Cell 69, 1237.
Oliner, J. D., et al. 1992. Nature 358, 80.
Fakharzadeh, S. S., et al. 1991. EMBO J. 10, 1565.
Product Information
FormLiquid
FormulationIn buffer, 50% glycerol.
PreservativeNone
Applications
Application NotesImmunoblotting (see below)
Immunofluorescence (see below)
Immunoprecipitation (see below)
Paraffin Sections (see below)
Application CommentsAntibodies should be titrated for optimal results in individual systems.
Biological Information
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing intitial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
ASK26 0

Documentation

MDM2 Antibody Sampler Kit-Human Certificates of Analysis

TitleLot Number
ASK26

References

Hivatkozások áttekintése
Marchetti, A., et al. 1995. J. Pathol. 175, 31.
Barak, Y., et al. 1993. EMBO J. 12, 461.
Ladanyi, M., et al. 1993. Cancer Res. 53, 16.
Leach, F. S., et al. 1993. Cancer Res. 53, 2231.
Oliner, J. D., et al. 1993. Nature 362, 857.
Momand, J., et al. 1992. Cell 69, 1237.
Oliner, J. D., et al. 1992. Nature 358, 80.
Fakharzadeh, S. S., et al. 1991. EMBO J. 10, 1565.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision26-February-2008 JSW
SynonymsAnti-Murine Double Minute Chromosome-2
ApplicationImmunoblotting (see below)
Immunofluorescence (see below)
Immunoprecipitation (see below)
Paraffin Sections (see below)
Application Data
DescriptionSet of 3 purified mouse monoclonal antibodies that recognize MDM2.
BackgroundIncreased expression of a gene termed MDM2, first identified in mouse as a small acentromeric extra-chromosomal element, was shown to become oncogenic upon amplification and tumorigenic when over-expressed in NIH3T3 and Rat2 cells. The human homolog of MDM2 gene has been isolated and mapped to chromosome 12q13-q14, a region that is found to be amplified in human sarcomas. Sequence analysis of the murine, rat, and human MDM2 genes suggests that MDM2 may be involved in transcriptional regulation in part by forming a stable complex with p53. Over-expression of MDM2 inhibits the ability of wild type p53 to stimulate expression of target genes, and this inhibition appears to result from MDM2 binding to the acidic activation domain of p53, thereby preventing p53 from directly contacting the transcriptional machinery. Interestingly, wild type p53 appears to positively regulate expression of the MDM2 gene. MDM2 amplification has been observed in sarcomas, and direct analysis of the MDM2 and p53 genes in sarcomas indicates that one or the other but not both of these genes is mutated in 70% of the tumors.
Specieshuman
FormLiquid
FormulationIn buffer, 50% glycerol.
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsAntibodies should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing intitial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesMarchetti, A., et al. 1995. J. Pathol. 175, 31.
Barak, Y., et al. 1993. EMBO J. 12, 461.
Ladanyi, M., et al. 1993. Cancer Res. 53, 16.
Leach, F. S., et al. 1993. Cancer Res. 53, 2231.
Oliner, J. D., et al. 1993. Nature 362, 857.
Momand, J., et al. 1992. Cell 69, 1237.
Oliner, J. D., et al. 1992. Nature 358, 80.
Fakharzadeh, S. S., et al. 1991. EMBO J. 10, 1565.