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14-536 MAP Kinase 2/Erk2 Protein, inactive, Human, 50 µg

Inactive, N-terminal GST-tagged, recombinant human full length MAP Kinase 2, for use in Kinase Assays.

MAP Kinase 2/Erk2 Protein, inactive, Human, 50 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-536
50 µg  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Catalogue Number14-536
Brand Family Upstate
Trade Name
  • Upstate
DescriptionMAP Kinase 2/Erk2 Protein, inactive, Human, 50 µg
OverviewN-terminal GST-tagged, recombinant human full length MAP Kinase 2
References
Product Information
Quality LevelMQ100
Applications
ApplicationInactive, N-terminal GST-tagged, recombinant human full length MAP Kinase 2, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceExpressed in E. coli
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
Gene Symbol
  • MAPK1
  • p40
  • PRKM2
  • MAPK2
  • PRKM1
  • p42-MAPK
  • p38
  • p41
  • ERK
  • ERK-2
  • ERT1
  • P42MAPK
  • p41mapk
  • ERK2
Protein TargetMAPK2
Purification MethodGlutathione agarose affinity chromatography
Target Sub-FamilyCMGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P28482 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
COFACTOR: Magnesium (By similarity).
SIZE: 360 amino acids; 41390 Da
SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine- kinase activity. Interacts with HSF4.
DOMAIN: SwissProt: P28482 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme.
SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
Molecular Weight67.8kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of MBP substrate
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -20°C
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
14-536 04053252622144

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Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases