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SCM064SFM LX-2 Human Hepatic Stellate Serum-Free Media

Serum-Free media optimized to expand and culture LX-2 human hepatic stellate cells.

ÚjSCM064SFM
250 mL  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Catalogue NumberSCM064SFM
DescriptionLX-2 Human Hepatic Stellate Serum-Free Media
Alternate Names
  • HSC
  • Perisinusoidal cells
  • Ito cells
  • Hepatic lipocytes
  • Hepatic pericytes
Background InformationLX-2 Human Hepatic Stellate Serum-Free Media (SCM064SFM) is a ready to use, serum-free culture medium specifically designed for the growth and proliferation of LX-2 human hepatic stellate cells (SCC064, SCC064SF).

Hepatic stellate cells are a major cell type responsible for liver fibrosis following their activation into fibrogenic myofibroblast-like cells in diseases such as chronic alcoholism, hepatitis B and C, fatty liver disease, obesity, and diabetes. LX-2 cells, an immortalized human hepatic stellate cells, retain key features of primary hepatic stellate cells (HSCs) and have been extensively utilized in the study of liver fibrosis.

SCM064SFM eliminates the reliance on FBS, providing a consistent and controlled environment for cell culture. This ensures reproducibility in experimental results and reduces variability often associated with serum-based media. The media is optimized to support the growth and proliferation of LX-2 cells, enabling researchers to conduct experiments with similar cell yields and viability compared with cells grown on FBS supplemented media. By eliminating the need for FBS, SCM064SFM significantly reduces the demand for animal-derived products, contributing to ethical research practices and animal welfare.
References
Product Information
Components
  • 1) 250 mL LX-2 Human Hepatic Stellate Serum-Free Media: (Catalog No. SCC064SFM). Store in liquid nitrogen.
Quality LevelMQ100
Applications
ApplicationSerum-Free media optimized to expand and culture LX-2 human hepatic stellate cells.
Key Applications
  • Stem Cell Culture
  • Cell Culture
Biological Information
HostHuman
Cell Line Type
  • Hepatocyte Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceAppearance (Color): Clear, Red Liquid
pH: 7.2 – 7.8
Osmolality: 289-310 mOsm
Sterility Tested: No Growth/Pass
Endotoxin: <2 EU/mL
Mycoplasma: Negative
Functional Assay: Thaw and culture of LX-2 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsUpon receipt store at -20˚C. When ready to use, thaw overnight at 2-8˚C. Once thawed, mix thoroughly. Use immediately and store at 2-8˚C for up to 3-4 weeks. Multiple freeze/thaw cycles are not recommended.
Packaging Information
Material Size250 mL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
SCM064SFM 04065272306232