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14-918M JAK1 Protein, active, 250 µg

Active, N-terminal GST-tagged, recombinant, human JAK1, amino acids 866-end, expressed by baculovirus in Sf21 insect cells. Activated in vitro by auto-phosphorylation. For use in Kinase Assays.

JAK1 Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-918M
250 µg  Also available in 10μg size as catalog # 14-918 and in bulk as catalog # 14-918-K.
Purchase on Sigma-Aldrich

Áttekintés

Description
Catalogue Number14-918M
Trade Name
  • Upstate
DescriptionJAK1 Protein, active, 250 µg
OverviewJanus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. JAK1 is involved in the interferon-alpha/beta and -gamma signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-alpha pathway, and between JAK1 and JAK2 in the interferon-gamma pathway, may reflect a requirement for these kinases in the correct assembly of interferon receptor complexes. These kinases couple cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and of a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs.
Product Information
Presentation50mM Tris/HCl pH7.5, 150mM NaCl, 5mM β-glycerophosphate, 0.5mM sodium orthovanadate, 50% v/v glycerol, 0.02% Triton® X-100, 0.5mM EDTA, 2mM DTT. Frozen solution.
Quality LevelMQ100
Applications
ApplicationActive, N-terminal GST-tagged, recombinant, human JAK1, amino acids 866-end, expressed by baculovirus in Sf21 insect cells. Activated in vitro by auto-phosphorylation. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceN-terminal GST-tagged, recombinant, human JAK1 amino acids 866-end, expressed by baculovirus in Sf21 insect cells. Activated in vitro by auto-phosphorylation.
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Gene Symbol
  • JAK1
  • JTK3
  • JAK1A
  • JAK1B
Purification MethodPurified using glutathione sepharose.
Target Sub-FamilyTK
UniProt Number
Molecular Weight61 kDa
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of peptide GEEPLYWSFPAKKK.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10μg size as catalog # 14-918 and in bulk as catalog # 14-918-K.
Global Trade Item Number
Katalógusszám GTIN
14-918M 04053252825446