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444279 InnoZyme™ Gelatinase Substrate, Fluorogenic

InnoZyme™ Gelatinase Substrate, Fluorogenic MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Gelatinase A/Gelatinase B Substrate III, α1(V)436-447 fTHP, H-(GPHyp)₅-GPK(Mca)-GPPG~VVGEK(Dnp)-GEQ-(GPHyp)₅-NH₂

444279
  
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Áttekintés

Replacement Information
Description
Overview

This product has been discontinued.





An internally quenched fluorogenic peptide substrate that undergoes selective hydrolysis by MMP-2 and MMP-9 (kcat/KM = 14,002 s-1M-1 and 5,499 s-1M-1, respectively) at the Gly~Val bond. The peptide substrate is designed from the five triple-helical collagen sequence hydrolyzed by MMP-9. Very weakly recognizes MMP-1, MMP-3, MMP-13, and MMP-14 activities (kcat/KM < 600 s-1M-1). Displays thermal stability and aqueous solubility. Also useful for continuously monitoring MMP triple helical peptidase (THP) activities.
Catalogue Number444279
Brand Family Calbiochem®
SynonymsGelatinase A/Gelatinase B Substrate III, α1(V)436-447 fTHP, H-(GPHyp)₅-GPK(Mca)-GPPG~VVGEK(Dnp)-GEQ-(GPHyp)₅-NH₂
References
ReferencesLauer-Fields, J.L., et al. 2003. J. Biol. Chem. 278, 18140.
Product Information
FormYellow solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₂₀₀H₂₈₃N₅₁O₆₈
Chemical formulaC₂₀₀H₂₈₃N₅₁O₆₈
Hygroscopic Hygroscopic
Applications
Biological Information
Purity≥95% by HPLC
Physicochemical Information
Peptide SequenceH₂N-(Gly-Pro-Hyp)₅-Gly-Pro-Lys(Mca)-Gly-Pro-Pro-Gly~Val-Val-Gly-Glu-Lys(Dnp)-Gly-Glu-Gln-(Gly-Pro-Hyp)₅-NH₂
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
444279 0

Documentation

InnoZyme™ Gelatinase Substrate, Fluorogenic Certificates of Analysis

TitleLot Number
444279

References

Hivatkozások áttekintése
Lauer-Fields, J.L., et al. 2003. J. Biol. Chem. 278, 18140.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-August-2008 RFH
SynonymsGelatinase A/Gelatinase B Substrate III, α1(V)436-447 fTHP, H-(GPHyp)₅-GPK(Mca)-GPPG~VVGEK(Dnp)-GEQ-(GPHyp)₅-NH₂
DescriptionAn internally quenched fluorogenic peptide substrate that undergoes selective hydrolysis by MMP-2 and MMP-9 (kcat/Km = 14,002 s-1M-1 and 5,499 s-1M-1, respectively) at the Gly-Val bond. The peptide substrate is designed from the five triple-helical collagen sequence hydrolyzed by MMP-9. Weakly cleaved by MMP-1, MMP-3, MMP-13, and MMP-14 (kcat/Km < 600 s-1M-1). Displays suitable thermal stability and aqueous solubility. Also useful for continuously monitoring MMP triple helical peptidase (THP) activity.
FormYellow solid
FormulationSupplied as a trifluoroacetate salt.
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₂₀₀H₂₈₃N₅₁O₆₈
Peptide SequenceH₂N-(Gly-Pro-Hyp)₅-Gly-Pro-Lys(Mca)-Gly-Pro-Pro-Gly~Val-Val-Gly-Glu-Lys(Dnp)-Gly-Glu-Gln-(Gly-Pro-Hyp)₅-NH₂
Purity≥95% by HPLC
SolubilityDMSO (5 mg/ml)
Storage Protect from light
-20°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesLauer-Fields, J.L., et al. 2003. J. Biol. Chem. 278, 18140.