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QIA129 InnoCyte™ Cell Invasion Assay Kit (24-well)

InnoCyte™ Cell Invasion Assay Kit (24-well) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
QIA129
  
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Áttekintés

Kulcsspecifikációk táblázata

Detection Methods
Fluorescence
Description
Overview

This product has been discontinued.



We are offering QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric (Cat. No. ECM554) as a possible alternative. Please read the alternative product documentation carefully and contact technical service if you need additional information.






A convenient and useful assay for the quantitative measurement of cell invasion. Contains 12 cell culture inserts with an 8 µm polycarbonate membrane. This membrane is coated with a thin layer of a biological matrix. This layer prevents noninvasive cells from going through the membrane. Cells are quantified utilizing a highly sensitive fluorescent dye (Excitation: ~485 nm; Emission: ~520 nm).
Catalogue NumberQIA129
Brand Family Calbiochem®
Materials Required but Not Delivered• Pipettors with disposable tips
• 96-well plate or strips in black or white color
• Fluorescence plate reader
• Tissue culture equipment
• Forceps
• Serum-free tissue culture medium
• Tissue culture medium with 10% FCS
References
ReferencesMontell, D.J. 2003. Nat. Rev. Mol. Cell Biol. 4, 13.
Egblad, M., et al. 2002. Nat. Rev. Cancer 2, 161.
Hood, J.D., et al. 2002. Nat. Rev. Cancer 2, 91.
Chatterjee, N., et al. 2001. Environ. Pathol. Toxicol. Oncol. 20, 211.
Koul, D., et al. 2001. Oncogene 20, 6669.
Kleinman, H.K., et al. In Molecular and Cellular Aspects of Basement Membranes, Rohrbach, D.H. and Timpl, R., eds. Academic Press, 1993, pp. 309.
Repesh, L.A. 1989. Invasion Metastasis 9, 192.
Albini, A., et al. 1987. Cancer Res. 47, 3229.
Terranova, V.P., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 465.
Liotta, L.A., 1984. Am. J. Pathol. 117, 339.
Product Information
Detection methodFluorescence
Form12 Tests
Format24-well plate
Kit contains24-Well Plate containing 12 cell culture inserts coated with Basement Membrane Extract, Cell Detachment Solution, Cell Staining Solution, Cell Staining Diluent, and a user protocol.
Quality LevelMQ100
Biological Information
Assay time24-48 h
Sample TypeCulture cells
Product Usage Statements
Intended useThe Calbiochem® InnoCyte™ Cell Invasion Assay is a quantitative assay for the study of tumor cell invasion in vitro. The assay is useful for discriminating between invasive and non-invasive tumor cell lines and for screening anti-cancer drugs that affect cancer cell invasion. Cell invasion is quantified by labeling the invaded cells with calcein-AM.
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage Multiple storage requirements
Storage ConditionsUpon arrival store the Calcein-AM at -20°C and the remaining components at 4°C.
Do not freeze Ok to freeze
Supplemental Information
Kit contains24-Well Plate containing 12 cell culture inserts coated with Basement Membrane Extract, Cell Detachment Solution, Cell Staining Solution, Cell Staining Diluent, and a user protocol.
Global Trade Item Number
Katalógusszám GTIN
QIA129 0