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14-485-D IKKβ

Recombinant human IKKβ, also known as IKK2, IKBKB, IKKB, and NFKBIKB, full-length expressed in a baculovirus-infected Sf9 insect cell expression system, for use in enzyme kinetics, screening inhibitors, and selectivity profiling.

IKKβ MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-485-D
10 μg  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Catalogue Number14-485-D
Brand Family Upstate
Trade Name
  • Upstate
DescriptionIKKβ
OverviewRecombinant human IKKβ, also known as IKK2, IKBKB, IKKB, and NFKBIKB, full-length expressed in a baculovirus-infected Sf9 insect cell expression system
References
Product Information
Presentation50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutatione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol
Quality LevelMQ100
Applications
ApplicationRecombinant human IKKβ, also known as IKK2, IKBKB, IKKB, and NFKBIKB, full-length expressed in a baculovirus-infected Sf9 insect cell expression system, for use in enzyme kinetics, screening inhibitors, and selectivity profiling.
Key Applications
  • Kinase Assay
Biological Information
Concentration0.1 mg/ml
Purity≥70%
SourceSf9 insect cells
Specific Activity58 pmol/min/µg
Entrez Gene Number
Entrez Gene SummaryNFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]
Gene Symbol
  • IKBKB
  • IKK-B
  • IKK-beta
  • FLJ40509
  • IKKB
  • IkBKB
  • NFKBIKB
  • MGC131801
  • IKK2
Protein TargetIKKβ
Target Sub-FamilyMisc
UniProt Number
UniProt SummaryFUNCTION: SwissProt: O14920 # Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3 (By similarity).
SIZE: 756 amino acids; 86564 Da
SUBUNIT: Preferentially found as a heterodimer with IKK-alpha but also as a homodimer. Directly interacts with IKK-gamma/NEMO and TRPC4AP. The tripartite complex can also bind to MEKK1, MAP3K14/NIK, IKAP and IKB-alpha-p65-p50 complex. Phosphorylated IKB-alpha is further released from the complex. Found in a complex composed of NCOA2, NCOA3, IKKA, IKBKG and CREBBP. Interacts with SQSTM1 through PRKCZ or PRKCI. Forms an NGF-induced complex with IKBKB, PRKCI and TRAF6. May interact with MAVS/IPS1. Interacts with NALP2. Interacts with Yersinia yopJ.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
PTM: Upon cytokine stimulation, phosphorylated on Ser-177 and Ser- 181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response. & Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, which blocks the I-kappa-B signaling pathway.
SIMILARITY: SwissProt: O14920 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. IkappaB kinase subfamily. & Contains 1 protein kinase domain.
Molecular Weight105 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Kinase assay: 10 µl of varying amounts of IKKβ was mixed with 5 µl of 1 mg/ml IKKtide synthetic peptide substrate and 5 µl of distilled water. Reaction was initiated by adding 5 µl of 250 µM [33P]-ATP and incubating for 15 min at 30°C followed by spotting the reaction on phosphocellulose paper, fixing in 1% phosphoric acid, and assaying with a scintillation counter.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for > 6 month at –80°C from date of receipt.
Packaging Information
Material Size10 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
14-485-D 04055977276596