Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

EZHIASF-14K Human Insulin ELISA, animal serum free

This Human Insulin ELISA, animal serum free is used to measure & quantify Insulin levels in Metabolism & Endocrine research.

Human Insulin ELISA, animal serum free MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: ELISA Detection Methods: Fluorescent, Colorimetric Research Category: Metabolism Research Sub-Category: Obesity, Metabolic Disorders
EZHIASF-14K
96-well strip plate  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Insulin HELISAFluorescent Colorimetric
Description
Catalogue NumberEZHIASF-14K
DescriptionHuman Insulin ELISA, animal serum free
Background InformationThis unique Human Insulin Elisa kit is animal serum free–the monoclonal antibodies are harvested from clones grown in culture medium devoid of any animal protein.
References
Product Information
Detection methodFluorescent Colorimetric
Precision, %
  • Inter-assay: Coefficient of Variation:6.0–7.1
  • Intra-assay: Coefficient of Variation: 1.7–1.9
Quality LevelMQ200
Applications
ApplicationThis Human Insulin ELISA, animal serum free is used to measure & quantify Insulin levels in Metabolism & Endocrine research.
Key Applications
  • ELISA
Application Notes3.5 hour assay, room temperature, 20 µL sample volume
Biological Information
SpecificityNo Cross-reactivity with Human Pro-insulin or Human C-peptide
Species Reactivity
  • Human
Analytes Available
  • Insulin
Entrez Gene Number
Entrez Gene SummaryAfter removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.
Gene Symbol
  • INS
  • proinsulin
  • insulin
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P01308 # Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
SIZE: 110 amino acids; 11981 Da
SUBUNIT: Heterodimer of a B chain and an A chain linked by two disulfide bonds.
SUBCELLULAR LOCATION: Secreted.
DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].
SIMILARITY: SwissProt: P01308 ## Belongs to the insulin family.
Accuracy
  • 88–128%
Physicochemical Information
Sensitivity
  • 0.73 µU/mL
Linearity of Dilution73–100%
Standard Curve Range
  • 2–200 µU/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • For research use only. Not for use in diagnostic procedures.
Storage and Shipping Information
Packaging Information
Material Size96-well strip plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
EZHIASF-14K 04053252588013

Kapcsolódó termékek és alkalmazások

Related Products

Katalógusszám Leírás
EZHI-14K Human Insulin ELISA
EZRMI-13K Rat/Mouse Insulin ELISA
HADK2MAG-61K MILLIPLEX MAP Human Adipokine Magnetic Bead Panel 2 - Endocrine Multiplex Assay

Kategóriák

Life Science Research > Antibodies and Assays > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits
Life Science Research > Protein Detection and Quantification > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits