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PF048 Heregulin, Human, Recombinant, E. coli

Heregulin, Human, Recombinant, E. coli MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Neu Differentiation Marker, NDF

PF048
  
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Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Purity
≥97% by SDS-PAGE
Description
Overview

This product has been discontinued.



Recombinant, human heregulin expressed in E. coli. Heregulins have been shown to be mitogenic for Schwann cells and various epithelial cells. It is recommended that the protein be titrated for optimal results in individual systems.
Catalogue NumberPF048
Brand Family Calbiochem®
SynonymsNeu Differentiation Marker, NDF
References
ReferencesRiese, D.J., et al. 1996. J. Biol. Chem. 271, 20047.
Holmes, W.E., et al. 1992. Science 256, 1205.
Karey, K.P., et al. 1988. Cancer Res. 48, 4083.
Product Information
FormLyophilized
FormulationLyophilized from PBS, 50 µg BSA/µg cytokine.
Quality LevelMQ100
Applications
Adatbeállítási hiba. A pagelet <collection_feature_application_id-PROLIF> nem található.
Application NotesProliferation Studies
Application CommentsIt is recommended that the protein be titrated for optimal results in individual systems.
Biological Information
Biological activityEC₅₀ ~20-40 ng/ml as determined by a cell proliferation assay using breast carcinoma cell line SK-BR-3 or in a serum-free cell proliferation assay using the human cell line MCF7.
Purity≥97% by SDS-PAGE
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsStore lyophilized protein dessicated at -20°C. Lyophilized product should be reconstituted in sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin to prepare a stock solution of no less than 50 µg/ml. Following initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
PF048 0

Documentation

Heregulin, Human, Recombinant, E. coli MSDS

Title

Safety Data Sheet (SDS) 

Heregulin, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
PF048

References

Hivatkozások áttekintése
Riese, D.J., et al. 1996. J. Biol. Chem. 271, 20047.
Holmes, W.E., et al. 1992. Science 256, 1205.
Karey, K.P., et al. 1988. Cancer Res. 48, 4083.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-September-2008 RFH
SynonymsNeu Differentiation Marker, NDF
ApplicationProliferation Studies
DescriptionRecombinant, human heregulin expressed in E. coli. Human recombinant heregulin whose DNA sequence encoding the EGF domain of heregulin α (amino acids 177-241) expressed in and purified from E. coli. The expressed protein consists of 65 amino acids with a predicted molecular weight of approximately 7 kDa.
BackgroundIn addition to the well-studied neurotrophic factors and their receptors, many other growth factors and cytokines have been identified which exhibit proliferative or neuroprotective effects. Heregulin is one member of a family of structurally related polypeptides, which includes the epidermal growth factor, transforming growth factor-alpha, cripto-1, amphiregulin, and several viral growth factors. Heregulin has been shown to mediate growth regulatory functions through the epidermal growth factor receptor.
FormLyophilized
FormulationLyophilized from PBS, 50 µg BSA/µg cytokine.
Purity≥97% by SDS-PAGE
Biological activityEC₅₀ ~20-40 ng/ml as determined by a cell proliferation assay using breast carcinoma cell line SK-BR-3 or in a serum-free cell proliferation assay using the human cell line MCF7.
CommentsIt is recommended that the protein be titrated for optimal results in individual systems.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsStore lyophilized protein dessicated at -20°C. Lyophilized product should be reconstituted in sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin to prepare a stock solution of no less than 50 µg/ml. Following initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesRiese, D.J., et al. 1996. J. Biol. Chem. 271, 20047.
Holmes, W.E., et al. 1992. Science 256, 1205.
Karey, K.P., et al. 1988. Cancer Res. 48, 4083.