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14-623M HIPK2 Protein, active, 250 µg

Active, N-terminal His6-tagged, recombinant, human HIPK2, amino acids 165-564, for use in Kinase Assays.

14-623M
250 µg  Also available in 10 μg size (cat#14-623) and in bulk (cat#14-623-K).
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Catalogue Number14-623M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionHIPK2 Protein, active, 250 µg
OverviewN-terminal His6-tagged, recombinant, human HIPK2, amino acids 165-564
References
Product Information
Quality LevelMQ300
Applications
ApplicationActive, N-terminal His6-tagged, recombinant, human HIPK2, amino acids 165-564, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourcebaculovirus in Sf21 insect cells
Entrez Gene Number
Entrez Gene SummaryHIPK2 is a conserved serine/threonine nuclear kinase that interacts with homeodomain transcription factors.[supplied by OMIM]
Gene Symbol
  • HIPK2
  • PRO0593
  • hHIPk2
Protein TargetHIPK2
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyCMGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q9H2X6 # Protein kinase acting as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. Inhibits cell growth and promotes apoptosis. Involved in transcriptional activation of TP53 and TP73. Phosphorylation of TP53 may be mediated by a TP53-HIPK2-AXIN1 complex. In response to TGFB, cooperates with DAXX to activate JNK. Phosphorylates the antiapoptotic factor CTBP1 and promotes its proteasomal degradation. In the Wnt/beta-catenin signaling pathway acts as an intermediate kinase between TAK1 and NLK to promote the proteasomal degradation of MYB (By similarity). Phosphorylates CBX4 upon DNA damage and promotes its E3 SUMO-protein ligase activity.
SIZE: 1198 amino acids; 130966 Da
SUBUNIT: Interacts with TRADD, TP53, TP73, TP63, CREBBP, DAXX, P53DINP1, SKI, SMAD1, SMAD2 and SMAD3, but not SMAD4. Interacts with NKX1-2, NKX2-5, SPN/CD43, UBE2I, HMGA1, CTBP1, AXIN1, NLK, MYB, POU4F1, POU4F2, POU4F3, UBE2I, UBL1. Probably part of a complex consisting of TP53, HIPK2 and AXIN1 (By similarity). Interacts with CBX4.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Concentrated in PML/POD/ND10 nuclear bodies. Small amounts are cytoplasmic.TISSUE SPECIFICITY: Highly expressed in heart, muscle and kidney. Weakly expressed in a ubiquitous way. Down-regulated in several thyroid and breast tumors.
PTM: Phosphorylated on tyrosines (By similarity). Autophosphorylated. & Sumoylated. When conjugated it is directed to nuclear speckles. Desumoylated by SENP1 (By similarity). Sumoylation on Lys-32 is promoted by the E3 SUMO-protein ligase CBX4.
SIMILARITY: SwissProt: Q9H2X6 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily. & Contains 1 protein kinase domain.
Molecular Weight49.6kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of 0.33mg/ml MBP (Catalog # 13-104)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-623) and in bulk (cat#14-623-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
14-623M 04053252924422

Documentation

Kapcsolódó termékek és alkalmazások

Alternative Packsize

Katalógusszám Leírás
14-623 HIPK2 Protein, active, 10 µg

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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Kategóriák

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases