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362280 Glycoprotein Deglycosylation Kit

362280
Purchase on Sigma-Aldrich

Áttekintés

Products

KatalógusszámCsomagolás Menny./csomag
362280-1KIT Kartondoboz 1 kit
Description
OverviewKit contains all enzymes and reagents needed to remove all N-linked, all simple O-linked, and virtually all-complex O-linked oligosaccharides from glycoproteins in a single reaction at neutral pH. Suitable for use with native or denaturation protocols, with no degradation of protein. This kit has been assembled to contain sequencing grade enzymes without azide, BSA, or glycerol. Each kit can be used to deglycosylate up to 2 mg glycoprotein.
Catalogue Number362280
Brand Family Calbiochem®
References
ReferencesChiba, A., et al. 1997. J. Biol. Chem. 272, 2156.
Jiang, M.S., and Hart, G.W. 1997. J. Biol. Chem. 272, 2421.
Wilkins, P.P., et al. 1996. J. Biol. Chem. 271, 18732.
Altmann, F., et al. 1995. Glycoconj. J. 12, 84.
Szkudlinski, M.W., et al. 1995.
Endocrinology 136, 3325.
Pahlsson, P., et al. 1994. Glycoconj. J. 11, 43.
Saito, S., et al. 1994. J. Biol. Chem. 269, 5644.
Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
Iwase, H., Hotta, K. 1993. Methods. Mol. Biol. 14, 151.
Stults, N.L., and Cummings, R.D. 1993. Glycobiology 3, 589.
Hard, K., et al. 1992. Eur. J. Biochem. 205, 785.
Kuraya, N., and Hase, S. 1992. J. Biochem. (Tokyo) 112, 122.
Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.
Fukuda, M., et al. 1987. J. Biol. Chem. 262, 11952.
Sojar, H.T., and Bahl, O.P. 1987. Arch. Biochem. Biophys. 259, 52.
Taga, E.M., et al. 1984. Biochemistry 23, 815.
Kobata, A. 1979. Anal. Biochem. 100, 1.
Uchida, Y., et al. 1979. J. Biochem (Tokyo) 86, 1573.
Glasgow, L.R., et al. 1977. J. Biol. Chem. 252, 8615.
Spiro, R.G., and Bhoyroo, V.D. 1974. J. Biol. Chem. 249, 5704.
Product Information
Kit containsBovine Fetuin Control, Denaturation Solution, Endo-α-N-Acetylgalactosaminidase, β1,4-Galactosidase, Glucosaminidase, N-Glycosidase F, Neuraminidase, Reaction Buffer, TRITON® X-100 Detergent, and a user protocol.
Quality LevelMQ100
Biological Information
Assay time3 h-5 days
Safety Information
R PhraseR: 20/22-24-36/37/38-41-42/43

Harmful by inhalation and if swallowed.
Toxic in contact with skin.
Irritating to eyes, respiratory system and skin.
Risk of serious damage to eyes.
May cause sensitization by inhalation and skin contact.
S PhraseS: 26-36/37/39-45-23

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Do not breathe fumes.
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage +2°C to +8°C
Do not freeze Yes
Supplemental Information
Kit containsBovine Fetuin Control, Denaturation Solution, Endo-α-N-Acetylgalactosaminidase, β1,4-Galactosidase, Glucosaminidase, N-Glycosidase F, Neuraminidase, Reaction Buffer, TRITON® X-100 Detergent, and a user protocol.
Global Trade Item Number
Katalógusszám GTIN
362280-1KIT 04055977213652