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371739 G-Proteins, Bovine Brain, Functional

G-Proteins, Bovine Brain, Functional, is a mixture of purified pertussis toxin-sensitive G-proteins from bovine brain. Suitable for functional studies. Contains Goα, Giα-1, Giα-2, and Giα-3.

G-Proteins, Bovine Brain, Functional MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
371739
  
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Áttekintés

Replacement Information
Description
Overview

This product has been discontinued.



Mixture of purified pertussis toxin-sensitive G-proteins from bovine brain. Suitable for functional studies of G-proteins. Contains the following heterotrimeric G-proteins: Goα (4 - 5 μM), Giα-1 (1 - 2 μM), Giα-2 (1 - 2 μM), and Giα-3 (<1 μM). The βγ-subunit complex associated with these α-subunits represents the predominant form found in bovine brain. Characterized by GTPγS binding activity and by immunoblotting using subunit-specific antisera.
Catalogue Number371739
Brand Family Calbiochem®
References
ReferencesCasey, P., et al. 1991. Methods Enzymol. 195, 315.
Linder and Gilman, A.G. 1991. Methods Enzymol. 195, 207.
Goldsmith, P.P., et al. 1987. J. Biol. Chem. 262, 14,683.
Product Information
FormLiquid
FormulationIn 50 mM HEPES, 1 mM DTT, 1 mM EDTA, 0.1% LUBROL® Detergent, pH 7.6.
Applications
ApplicationG-Proteins, Bovine Brain, Functional, is a mixture of purified pertussis toxin-sensitive G-proteins from bovine brain. Suitable for functional studies. Contains Goα, Giα-1, Giα-2, and Giα-3.
Key Applications Immunoblotting (Western Blotting)
Application NotesSuitable for GTPγS binding assay and Immunoblotting
Application CommentsCharacterized by GTPγS binding and in immunoblot using subunit-specific antisera. Titration is recommended.
Biological Information
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C)
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
371739 0

Documentation

G-Proteins, Bovine Brain, Functional Certificates of Analysis

TitleLot Number
371739

References

Hivatkozások áttekintése
Casey, P., et al. 1991. Methods Enzymol. 195, 315.
Linder and Gilman, A.G. 1991. Methods Enzymol. 195, 207.
Goldsmith, P.P., et al. 1987. J. Biol. Chem. 262, 14,683.

Citations

Titulus
  • Rodrigo Cunha, M Dolores Constantino and J Alexandre Ribeiro. (1999) G Protein coupling of CGS 21680 binding sites in the rat hippocampus and cortex is different from that of adenosine A1 and striatal A2A receptors.. Naunyn-Schmiedeberg's Archives of Pharmacology 359, 295-302.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision02-August-2007 RFH
    ApplicationSuitable for GTPγS binding assay and Immunoblotting
    DescriptionMixture of purified pertussis toxin-sensitive G proteins from bovine brain useful for functional studies. Contains the following heterotrimeric G proteins: Goα (approximately 4-5 µM), Giα-1 (approximately 1-2 µM), Giα-2 (approximately 1-2 µM), and Giα-3 (<1 µM). The βγ subunit complex (approximately 8 µM) associated with these α subunits represents the predominant form found in bovine brain.
    FormLiquid
    FormulationIn 50 mM HEPES, 1 mM DTT, 1 mM EDTA, 0.1% LUBROL® Detergent, pH 7.6.
    Concentration Label Please refer to vial label for lot-specific concentration
    CommentsCharacterized by GTPγS binding and in immunoblot using subunit-specific antisera. Titration is recommended.
    Storage Avoid freeze/thaw
    ≤ -70°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C)
    Toxicity Standard Handling
    ReferencesCasey, P., et al. 1991. Methods Enzymol. 195, 315.
    Linder and Gilman, A.G. 1991. Methods Enzymol. 195, 207.
    Goldsmith, P.P., et al. 1987. J. Biol. Chem. 262, 14,683.
    Citation
  • Rodrigo Cunha, M Dolores Constantino and J Alexandre Ribeiro. (1999) G Protein coupling of CGS 21680 binding sites in the rat hippocampus and cortex is different from that of adenosine A1 and striatal A2A receptors.. Naunyn-Schmiedeberg's Archives of Pharmacology 359, 295-302.
    		•