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330655 Estrogen Receptorα, Human, Recombinant, S. frugiperda

Estrogen Receptorα, Human, Recombinant, S. frugiperda MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: ER-α

330655
  
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Áttekintés

Replacement Information
Description
Overview

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Recombinant, human estrogen receptor-α expressed in S. frugiperda insect cells. A hormone-inducible transcription factor that can positively or negatively regulate expression of many genes involved in tissue growth and differentiation. ER-α is essential for induction of the oxytocin receptor by estrogen. Activated ER-α is involved in the modulation of transcription triggered by AP-1. Provided in a soluble, functionally-active form. Binds to estradiol (Kd = 300 pM) and to a fluorescein-labeled estrogen response element (ERE; Kd = 10 nM). Suitable for in vitro transcription assays.
Catalogue Number330655
Brand Family Calbiochem®
SynonymsER-α
References
ReferencesTeyssier, C., et al. 2001. J. Biol. Chem. 276, 36361.
Young, L.T., et al. 1998. NeuroReport 9, 933.
Tzukeman, M.T., et al. 1994. Mol. Endocrinol. 8, 21.
Beekman, J.M., et al. 1993. Mol. Endocrinol. 7, 1266.
Obourn, J.D., et al. 1993. Biochemistry 32, 6229.
Product Information
ActivityBinds ≥ 960 pmol/[³H]-estradiol/ml
FormLiquid
FormulationIn 500 mM KCl, 50 mM Tris, 2 mM DTT, 1 mM EDTA, 1 mM sodium vanadate, 10% glycerol, 0.02% NaN₃, pH 7.5.
Applications
Biological Information
Purity≥80% by SDS-PAGE
Specific ActivityBinds ≥ 5,000 pmol [³H]-estradiol/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C). DO NOT VORTEX.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
330655 0

Documentation

Estrogen Receptorα, Human, Recombinant, S. frugiperda Certificates of Analysis

TitleLot Number
330655

References

Hivatkozások áttekintése
Teyssier, C., et al. 2001. J. Biol. Chem. 276, 36361.
Young, L.T., et al. 1998. NeuroReport 9, 933.
Tzukeman, M.T., et al. 1994. Mol. Endocrinol. 8, 21.
Beekman, J.M., et al. 1993. Mol. Endocrinol. 7, 1266.
Obourn, J.D., et al. 1993. Biochemistry 32, 6229.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision08-March-2011 JSW
SynonymsER-α
DescriptionRecombinant, human estrogen receptor-α expressed in S. frugiperda insect cells. A hormone-inducible transcription factor that can positively or negatively regulate the expression of many genes involved in tissue growth and differentiation. Provided in a soluble, functionally active form. Binds to estradiol (Kd = 300 pM) and to fluorescein-labeled estrogen response element (ERE; Kd = 10 nM). Suitable for in vitro transcription assays.
FormLiquid
FormulationIn 500 mM KCl, 50 mM Tris, 2 mM DTT, 1 mM EDTA, 1 mM sodium vanadate, 10% glycerol, 0.02% NaN₃, pH 7.5.
Purity≥80% by SDS-PAGE
Specific activityBinds ≥ 5,000 pmol [³H]-estradiol/mg protein
ActivityBinds ≥ 960 pmol/[³H]-estradiol/ml
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C). DO NOT VORTEX.
Toxicity Standard Handling
ReferencesTeyssier, C., et al. 2001. J. Biol. Chem. 276, 36361.
Young, L.T., et al. 1998. NeuroReport 9, 933.
Tzukeman, M.T., et al. 1994. Mol. Endocrinol. 8, 21.
Beekman, J.M., et al. 1993. Mol. Endocrinol. 7, 1266.
Obourn, J.D., et al. 1993. Biochemistry 32, 6229.