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324784 ErbB2, GST-Fusion, Human, Recombinant, S. frugiperda

ErbB2, GST-Fusion, Human, Recombinant, S. frugiperda MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Receptor tyrosine-protein kinase erbB-2, Tyrosine kinase-type cell surface receptor HER2, v-erb-b2 erythroblastic leukemia viral oncogene homolog 2

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Áttekintés

Replacement Information
Description
OverviewRecombinant, human ErbB2 fused at the N-terminus to a GST-thrombin cleavage site sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. ErbB2 is a component of a neuregulin-receptor complex that regulates neuromuscular synapse formation. It plays an important role in muscle spindle development. Overexpression of ErbB2 is reported in ~30% of all breast cancer cases. Amino acid sequence human ERB2 679-1255.
Catalogue Number324784
Brand Family Calbiochem®
SynonymsReceptor tyrosine-protein kinase erbB-2, Tyrosine kinase-type cell surface receptor HER2, v-erb-b2 erythroblastic leukemia viral oncogene homolog 2
References
ReferencesAmin, D.N., et al. 2004. Oncogene 23, 1428;. Vernimmen, D., et al. 2003. J. Cancer 89, 899. Ozcelik, C., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 8880. Coussens, L., et al. 1985 Science 230, 1132.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to Poly(Glu,Tyr)<sub>4:1</sub> per min at 30°C using variable concentrations of ATP (100 nM to 5.8 µM).
FormLiquid
FormulationIn 100 mM NaCl, 50 mM Tris-HCl, 15 mM reduced glutathione, 5 mM DTT, 20% glycerol, pH 8.0.
Applications
Biological Information
Specific Activity≥20 U/mg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
324784 0

Documentation

ErbB2, GST-Fusion, Human, Recombinant, S. frugiperda Certificates of Analysis

TitleLot Number
324784

References

Hivatkozások áttekintése
Amin, D.N., et al. 2004. Oncogene 23, 1428;. Vernimmen, D., et al. 2003. J. Cancer 89, 899. Ozcelik, C., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 8880. Coussens, L., et al. 1985 Science 230, 1132.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-January-2010 RFH
SynonymsReceptor tyrosine-protein kinase erbB-2, Tyrosine kinase-type cell surface receptor HER2, v-erb-b2 erythroblastic leukemia viral oncogene homolog 2
DescriptionRecombinant, human ErbB2 fused at the N-terminus to a GST-thrombin cleavage site sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. ErbB2 is a component of a neuregulin-receptor complex that regulates neuromuscular synapse formation. It plays an important role in muscle spindle development. Overexpression of ErbB2 is reported in ~30% of all breast cancer cases. Amino acid sequence human ERB2 679-1255.
FormLiquid
FormulationIn 100 mM NaCl, 50 mM Tris-HCl, 15 mM reduced glutathione, 5 mM DTT, 20% glycerol, pH 8.0.
Concentration Label Please refer to vial label for lot-specific concentration
Recommended reaction conditions
96-Well Membrane Binding Kinase Activity Assay Protocol:
Materials Required • Standard Kinase Assay Buffer: 125 mM HEPES-NaOH, 7.5 mM MgCl2, 7.5 mM MnCl2, 7.5 µM sodium -orthovanadate, 2.5 mM DTT, pH 7.5 • 10X Kinase Dilution Buffer: 500 mM HEPES-NaOH, 2.5 mg/ml PEG20.000, 10 mM DTT, pH 7.5 • Substrate: Poly(Glu,Tyr)4:1, 250 ng/50 µl final concentration • ErbB2, GST-Fusion, Human, Recombinant, S. frugiperda, diluted in 1X Kinase Dilution Buffer to desired concentration (we use 200 ng in a 50 µl reaction) • 33P-γ-ATP • ATP Mix: 1 µM ATP in H2O with 1 x 106 cpm/5 µl • 96-well V-bottom MTP (polypropylene) plates (Nunc Cat. No. 442587) • 96-well Multiscreen Vacuum Manifold (Millipore Cat. No. MAVM096OR) • 96-well Multiscreen Filterplates, phosphocellulose membrane (Millipore Cat. No. MSPH N6B50) and glass fiber (Millipore Cat. No. MSFC N6B50) • 2% H3PO4 • 0.9% NaCl
Activity Assay Protocol 1. In a polypropylene V-bottom plate add 20 µl Kinase Assay Buffer 2. If desired, add 5 µl test substance (e.g., inhibitor, activator, etc.; in 10% DMSO) 3. Add 10 µl Substrate (diluted in H2O). 4. Add 10 µl diluted ErbB2. 5. Add 5 µl ATP Mix. 6. Mix on a shaker and incubate at 30°C for 80 min. 7. Stop the reaction with 50 µl H3PO4. 8. Prewet the filter plate with 200 µl H2O. 9. Filter the reaction mix by applying vacuum. 10. Wash 3 times with 200 µl 0.9% NaCl. 11. Add 200 µl scintillation fluid to each well and count on a scintillation counter.
Specific activity≥20 U/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to Poly(Glu,Tyr)4:1 per min at 30°C using variable concentrations of ATP (100 nM to 5.8 µM).
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesAmin, D.N., et al. 2004. Oncogene 23, 1428;. Vernimmen, D., et al. 2003. J. Cancer 89, 899. Ozcelik, C., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 8880. Coussens, L., et al. 1985 Science 230, 1132.