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509230 ER Export Inhibitor II, FLI-06 - Calbiochem

A cell-permeable compound that reversibly blocks cellular glycosylation and surface expression of Notch, APP, Klotho by blocking events prior to cargo assembly at the ER exit sites.

ER Export Inhibitor II, FLI-06 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Cyclohexyl 2,7,7-trimethyl-4-(4-nitrophenyl)-5-oxo-1,4,5,6,7,8-hexahydroquinoline-3-carboxylate, Cyclohexyl 4-(4ʹ-nitrophenyl)-2,7,7-trimethyl-5-oxo-1,4,5,6,7,8-hexahydroquinoline-3-carboxylate, FLI06

Primary Target: ER
509230
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Empirical Formula
C₂₅H₃₀N₂O₅

Products

KatalógusszámCsomagolás Menny./csomag
5.09230.0001 Üvegpalack 10 mg
Description
OverviewA cell-permeable, non-toxic, dihydropyrimidine compound that prevents cellular glycosylation and surface expression of Notch, APP, Klotho (Effect. conc. = 10 µM in HEK293, HeLa, U2OS cultures) in a rapid and reversible manner by blocking events prior to cargo assembly at the ER exit sites (ERES) without affecting other post-ERES events targeted by BFA, GCA, Exo1, A5, Retro-2, U18666A, or Vacuolin-1 (Cat. nos. 203729, 345862, 341220, 444805, 554715, 662015, & 673000, respectively). Notch signaling is shown to be effectively inhibited by treatment with either FLI-06 or the γ-Secretase inhibitior DAPT (Cat. nos. 565770 & 565784) in both C2C12 cultures (10 µM FLI-06) in vitro and in zebrafish embryos (50 µM FLI-06) in vivo.
Catalogue Number509230
Brand Family Calbiochem®
SynonymsCyclohexyl 2,7,7-trimethyl-4-(4-nitrophenyl)-5-oxo-1,4,5,6,7,8-hexahydroquinoline-3-carboxylate, Cyclohexyl 4-(4ʹ-nitrophenyl)-2,7,7-trimethyl-5-oxo-1,4,5,6,7,8-hexahydroquinoline-3-carboxylate, FLI06
References
ReferencesKramer, A., et al. 2013. Nat. Chem. Biol. 9, 731.
Product Information
FormYellow powder
Hill FormulaC₂₅H₃₀N₂O₅
Chemical formulaC₂₅H₃₀N₂O₅
ReversibleY
Quality LevelMQ100
Applications
Biological Information
Primary TargetER
Purity≥95% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
5.09230.0001 04055977241723

Documentation

ER Export Inhibitor II, FLI-06 - Calbiochem MSDS

Title

Safety Data Sheet (SDS) 

References

Hivatkozások áttekintése
Kramer, A., et al. 2013. Nat. Chem. Biol. 9, 731.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision08-May-2014 JSW
SynonymsCyclohexyl 2,7,7-trimethyl-4-(4-nitrophenyl)-5-oxo-1,4,5,6,7,8-hexahydroquinoline-3-carboxylate, Cyclohexyl 4-(4ʹ-nitrophenyl)-2,7,7-trimethyl-5-oxo-1,4,5,6,7,8-hexahydroquinoline-3-carboxylate, FLI06
DescriptionA cell-permeable, non-toxic, dihydropyridine compound that prevents cellular maturation/glycosylation and surface expression of Notch, APP, Klotho (Effect. conc. = 10 µM in HEK293, HeLa, U2OS cultures) in a rapid and reversible manner by blocking events prior to cargo assembly at the endoplasmic reticulum exit sites (ERES) without affecting cytoskeleton, endocytosis, or other post-ERES events targeted by BFA, GCA, Exo1, A5, Retro-2, U18666A, or Vacuolin-1 (Cat. nos. 203729, 345862, 341220, 444805, 554715, 662015, & 673000, respectively). Notch signaling is shown to be effectively inhibited by treatment with either FLI-06 or the γ-Secretase inhibitior DAPT (Cat. nos. 565770 & 565784) in both C2C12 cultures (10 µM FLI-06) in vitro and in zebrafish embryos (50 µM FLI-06) in vivo.
FormYellow powder
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₂₅H₃₀N₂O₅
Purity≥95% by HPLC
SolubilityDMSO (100 mg/ml). Use only fresh DMSO for reconstitution.
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesKramer, A., et al. 2013. Nat. Chem. Biol. 9, 731.