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L2113-BC EDTA (Versen), 1 %

L2113-BC
  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
OverviewTrypsin/EDTA solutions (0.05 or 0.02% in PBS, without Ca2+, Mg2+) are used for passaging monolayer cultures. Trypsin affects the cell membrane and the cellular, and also the intercellular mucoproteins. Trypsin can be inhibited with the use of serum.
Specifications:

Functional Test: Pass
Activity Test: Pass
pH: 7.2-7.5
Osmolality: 0.27-0.30 Osm/kg H20
Color: Colorless
Appearance: Clear
Recommendation Instructions:

Recommendations Trypsin (1:250)/EDTA solutions:
Trypsin (1:250)/EDTA solutions (0.05/0.02 % in PBS without Ca2+, Mg2+) to passage monolayer cultures:
1. Wash cell culture (monolayer) using physiological saline (w/o Ca2+, Mg2+).
2. Apply a small quantity of Trypsin (1:250)/EDTA solution with a pipette to cell culture flask; a thin film is sufficient.
3. Incubate culture flask at +37°C for approx. 5 min.
4. Centrifuge cell suspension or transfer directly to a serum containing culture medium.

With higher Trypsin concentrations, it is recommended to trypsinate at +2-+8°C.
Catalogue NumberL2113-BC
Brand Family Calbiochem®
References
Product Information
Quality LevelMQ300
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Storage +2°C to +8°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
L2113-BC 0

Documentation