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CHR103 Digitonin solution- high purity

CHR103
5 X 1 mL  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Catalogue NumberCHR103
DescriptionDigitonin solution- high purity
Background InformationDigitonin is a non-ionic steroid saponin derived from Digitalis purpurea. Historically, digitonin has been used to solubilize receptors and permeabilize membranes for sample preparation and analysis. Digitonin interacts with cholesterol creating a digitonin-cholesterol complex, which leads to subsequent removal of cholesterol and cellular membrane permeabilization. Therefore, cellular compartments composed of low amounts of cholesterol are protected from digitonin effects, particularly the nuclear membrane.

Due to digitonin’s mechanism of action, many other epigenetic applications utilize digitonin for nuclear extraction. For example, assay for Transposase-Accessible Chromatin with high throughput sequencing (ATAC-Seq) is a valuable method to map open chromatin sites and assess the epigenetic landscape. In combination with other detergents, a low concentration of digitonin is employed to isolate the nuclear pellet followed by digestion of the nuclei with an increased concentration of digitonin5. In our ChIC (Chromatin Immuno-Cleavage) Kits (part #s: CHR100-102), digitonin is utilized for antibody targeting of micrococcal nuclease for chromatin tagging.
References
Product Information
Components
  • 5 x 1 ml 2% ( (weight/volume) Digitonin in DMSO.
PresentationEach vial contains 20mg high purity Digitonin disolved in 1ml DMSO.
Applications
Biological Information
Concentration2% Digitonin in DMSO
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions-20°C
Packaging Information
Material Size5 X 1 mL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
CHR103 04061841034650