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HTS075M ChemiScreen™ Membrane Preparation
Recombinant Human M5 Muscarinic Acetylcholine Receptor

Human M5 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.

ChemiScreen™ Membrane Preparation
Recombinant Human M5 Muscarinic Acetylcholine Receptor MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Host Cell: Chem-1 Uni Prot Number: P08912 Entrez Gene Number: NM_012125.2
HTS075M
200 units  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

SpeciesHost CellsProtein Target
Human Chem-1M5
Description
Catalogue NumberHTS075M
Brand Family Chemicon®
Trade Name
  • ChemiScreen
  • Chemicon
DescriptionChemiScreen™ Membrane Preparation
Recombinant Human M5 Muscarinic Acetylcholine Receptor
OverviewFull-length human CHRM5 cDNA, encoding M5
Background InformationThe muscarinic acetylcholine receptor family consists of five GPCRs that mediate some of the neurotransmission functions of acetylcholine in the CNS and the periphery. The M5 receptor, along with the M1 and M3 receptors, signal through Gq/11 and subsequent release of Ca++ from the ER (Caulfield and Birdsall, 1998). Although M5 is expressed in the CNS at relatively low levels, it appears to be the only muscarinic acetylcholine receptor expressed in midbrain dopaminergic neurons, and it has been shown to mediate dopamine release from these neurons. The M5 receptor is also expressed in blood vessels in the brain and the periphery, and studies with M5 knockout mice demonstrate that M5 mediates the vasodilatory action of acetylcholine on cerebral microvessels. Further studies with M5 knockout mice also indicate a role for M5 in the rewarding effects of cocaine and morphine (Wess, 2004). Chemicon's M5 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of M5 interactions with its ligands. The membrane preparations exhibit a Kd of 0.16 nM for [3H]-Scopolamine methyl chloride (NMS). With 10 μg/well M5 Membrane Prep and 1 nM [3H]-NMS, greater than 5-fold signal-to-background ratio was obtained.
References
Product Information
FormatMembranes
PresentationLiquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA no preservatives. Packaging method: Membranes protein were adjusted to 0.5 mg/mL in 1 mL packaging buffer, rapidly frozen, and stored at -80°C.
Quality LevelMQ100
Applications
ApplicationHuman M5 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
Key Applications
  • Radioligand binding assay
  • GTPγS binding
Application NotesRadioligand binding assay and GTPγS binding.
Biological Information
Entrez Gene Number
Entrez Gene SummaryThe muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, stimulation of this receptor is known to increase cyclic AMP levels.
Gene Symbol
  • CHRM5
  • MGC41838
  • HM5
GPCR ClassA
Host CellsChem-1
Protein TargetM5
Target Sub-FamilyAcetylcholine (muscarinic)
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P08912 # The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
SIZE: 532 amino acids; 60074 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: P08912 ## Belongs to the G-protein coupled receptor 1 family.
Incubation ConditionsMembranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted. Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C Radioligand: [3H] NMS (Perkin Elmer#:NEX-636 )
Wash Buffer: 50 mM Hepes, pH 7.4, 500 mM NaCl , 0.1% BSA, filtered and stored at 4°C.
One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 5-fold signal:background with 3H-labeled NMS at 1 nM
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceBmax: 2.49 pmol/mg; Kd: 0.16nM
1 unit = 10 μg
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain frozen at -70°C for up to 2 years. Do not freeze and thaw.
Packaging Information
Material Size200 units
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
HTS075M 04053252737954

Documentation

ChemiScreen™ Membrane Preparation
Recombinant Human M5 Muscarinic Acetylcholine Receptor MSDS

Title

Safety Data Sheet (SDS) 

References

Reference overviewPub Med ID
Muscarinic acetylcholine receptor knockout mice: novel phenotypes and clinical implications.
Wess, Jürgen
Annu. Rev. Pharmacol. Toxicol., 44: 423-50 (2004)  2004

Kivonat megmutatása 		14744253 14744253
International Union of Pharmacology. XVII. Classification of muscarinic acetylcholine receptors
Caulfield, M P and Birdsall, N J
Pharmacol Rev, 50:279-90 (1998)  1998

9647869 9647869

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Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Membrane Preparations
Life Science Research > Proteins and Enzymes > GPCR Membrane Preparations