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219497 Cathepsin L Substrate I, Fluorogenic - Calbiochem

A tetrapeptidyl fluorogenic substrate for human cathepsin L (kcat = 20.8 sec⁻¹, Km = 11.2 µM, and kcat/Km = 1.9 x 10⁶ M⁻¹ sec⁻¹).

Cathepsin L Substrate I, Fluorogenic - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Ac-HRYR-ACC

219497
  
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Áttekintés

Replacement Information
Description
OverviewA tetrapeptidyl fluorogenic substrate for human cathepsin L (kcat = 20.8 sec-1, Km = 11.2 µM, and kcat/Km = 1.9 x 106 M-1 sec-1). Displays ~270-fold and ~2,500-fold greater selectivities over cathepsin B (kcat/Km = 7.0 x 103 M-1 sec-1),respectively and cathepsin K (kcat/Km = 7.6 x 102 M-1 sec-1). An excitation wavelength of 380 nm and an emission wavelength of 460 nm is recommended in order to maximize the signal of the ACC group over the background signal of uncleaved substrate.

This product has been discontinued.
ACC = 7-Amino-4-carbamoylmethylcoumarin.
Catalogue Number219497
Brand Family Calbiochem®
SynonymsAc-HRYR-ACC
References
ReferencesChoe, Y., et al. 2006. J. Biol. Chem. 281, 12824.
Product Information
ATP CompetitiveN
FormLyophilized
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₄₀H₅₂N₁₄O₉
Chemical formulaC₄₀H₅₂N₁₄O₉
Hygroscopic Hygroscopic
ReversibleN
Applications
Biological Information
Primary TargetHuman cathepsin L
Primary Target IC<sub>50</sub>kcat = 20.8 sec⁻¹, Km = 11.2 µM, and kcat/Km = 1.9 x 106 M⁻¹ sec⁻¹ as substrate for human cathepsin LL
Purity≥95% by HPLC
Physicochemical Information
Cell permeableN
Peptide SequenceAc-His-Arg-Tyr-Arg-ACC
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
219497 0

Documentation

Cathepsin L Substrate I, Fluorogenic - Calbiochem Certificates of Analysis

TitleLot Number
219497

References

Hivatkozások áttekintése
Choe, Y., et al. 2006. J. Biol. Chem. 281, 12824.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-September-2007 RFH
SynonymsAc-HRYR-ACC
DescriptionA tetrapeptidyl fluorogenic substrate for human cathepsin L (kcat = 20.8 sec-1, Km = 11.2 µM, and kcat/Km = 1.9 x 106 M-1 sec-1). Displays ~270-fold and ~2,500-fold greater selectivities, respectively, over cathepsin B (kcat/Km = 7.0 x 103 M-1 sec-1) and cathepsin K (kcat/Km = 7.6 x 102 M-1 sec-1). An excitation wavelength of 380 nm and an emission wavelength of 460 nm is recommended in order to maximize the signal of the ACC group over the background signal of uncleaved substrate.
FormLyophilized
FormulationSupplied as a trifluoroacetate salt.
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₄₀H₅₂N₁₄O₉
Peptide SequenceAc-His-Arg-Tyr-Arg-ACC
Purity≥95% by HPLC
SolubilityDMSO (5 mg/ml) or 5% Acetic acid (5 mg/ml)
Storage Protect from light
-20°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesChoe, Y., et al. 2006. J. Biol. Chem. 281, 12824.