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CC126 Caspase 1, recombinant human active

Caspase 1, recombinant human active MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Activity Assay UniProt Number: P29466
CC126
25 units  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information

Kulcsspecifikációk táblázata

Key ApplicationsEntrez Gene NumberSpeciesUni Prot Number
ACTNM_001223.3 NM_033292.2 NM_033293.2 NM_033294.2 NM_033295.2 Human P29466
Description
Catalogue NumberCC126
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionCaspase 1, recombinant human active
OverviewCaspase-1 (also known as ICE, Interleukin-1beta-Converting Enzyme) is the prototypical member of the caspase family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa pro-enzyme. The pro-enzyme is matured by proteolysis to yield large (20 kD) and small (10 kD) subunits. The active enzyme is a heterotetramer consisting of two large and two small subunits. To date the mechanism of regulation of caspase-1 activation is complex and poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive pro-enzyme form.

The active recombinant human caspase-1 was expressed in E. coli. The expressed caspase-1 spontaneously undergoes autoprocessing to yield the subunits characteristic of the native enzyme. The recombinant caspase-1 preferentially cleaves the substrates consisting of consensus sequence YVAD (e.g., YVAD-AFC and YVAD-pNA). The recombinant enzyme can also be used as a positive control in caspase assays or in determining the specificity of caspase substrates.

Recommended usage: 0.5-1 unit for fluorometric caspase assays and 2-4 units for colorimetric caspase assays. Optimal working dilutions must be determined by end user.
References
Product Information
PresentationLyophilized. Reconstitute in 25 μL PBS containing 15% glycerol.
Quality LevelMQ100
Applications
Key Applications
  • Activity Assay
Biological Information
PurityPartially purified recombinant caspase 3 with a full length HIS-6-tag. Approximately 10-20% caspase 1 protein. The remainder is E.Coli proteins. Approximately 0.8-2 mg total protein/U of activity; E.Coli proteins have no reactivity.
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms.
Gene Symbol
  • CASP1
  • IL1BCE
  • P45
  • IL-1BC
  • IL1BC
  • p45
  • IL1B-convertase
  • CASP-1
  • ICE
  • EC 3.4.22.36 [Contains: Caspase-1 subunit p20
  • Caspase-1 subunit p10].
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P29466 # Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
SIZE: 404 amino acids; 45159 Da
SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
PTM: The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
SIMILARITY: SwissProt: P29466 ## Belongs to the peptidase C14 family. & Contains 1 CARD domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain unopened and rehydrated product at -70°C in undiluted, freeze-thaw protected, tightly sealed aliquots. Avoid repeated freeze/thaw cycles. Product stable for 6 months from date of purchased if handled appropriately.
Packaging Information
Material Size25 units
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
CC126 04053252281341

Documentation

Caspase 1, recombinant human active MSDS

Title

Safety Data Sheet (SDS) 

Caspase 1, recombinant human active Certificates of Analysis

TitleLot Number
HUMAN CASPASE-1 (ACTIVE) RECOMBINANT PROTEIN 3091681
HUMAN CASPASE-1 (ACTIVE) RECOMBINANT PROTEIN 2930545
HUMAN CASPASE-1 (ACTIVE) RECOMBINANT PROTEIN 3126999
HUMAN CASPASE-1 (ACTIVE) RECOMBINANT PROTEIN 3011535
HUMAN CASPASE-1 (ACTIVE) RECOMBINANT PROTEIN 3150924
HUMAN CASPASE-1 (ACTIVE) RECOMBINANT PROTEIN 3029801
HUMAN CASPASE-1 (ACTIVE) RECOMBINANT PROTEIN - 2464509 2464509
HUMAN CASPASE-1 (ACTIVE) RECOMBINANT PROTEIN - 2019754 2019754
HUMAN CASPASE-1 (ACTIVE) RECOMBINANT PROTEIN - 2042755 2042755
HUMAN CASPASE-1 (ACTIVE) RECOMBINANT PROTEIN - 2048773 2048773
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References

Reference overviewPub Med ID
NKp46 defines ovine cells that have characteristics corresponding to NK cells.
Timothy Connelley,Anne K Storset,Alan Pemberton,Niall Machugh,Jeremy Brown,Hege Lund,Ivan W Morrison
Veterinary research  42  2001

Kivonat megmutatása Teljes cikk		21345198 21345198

Data Sheet

Title
HUMAN CASPASE-1 (ACTIVE) RECOMBINANT PROTEIN