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12-414 CHKtide, biotin conjugate

CHKtide, biotin conjugate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
12-414
200 µg  
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Áttekintés

Description
Catalogue Number12-414
Brand Family Upstate
Trade Name
  • Upstate
DescriptionCHKtide, biotin conjugate
Product Information
Presentationsterile, distilled water
Quality LevelMQ100
Applications
Key Applications
  • Kinase Assay
Biological Information
Entrez Gene Number
Gene Symbol
  • CHEK1
  • CHK1
Protein TargetCHK
Target Sub-FamilyCAMK
UniProt Number
UniProt SummaryFUNCTION: SwissProt: O14757 # Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X- S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser- 293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK- cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation.


SIZE: 476 amino acids; 54420 Da

SUBUNIT: Interacts with BRCA1, CLSPN, PPM1D, RAD51, TIMELESS, XPO1/CRM1 and YWHAZ/14-3-3 zeta.

SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Nuclear export is mediated at least in part by XPO1/CRM1. Also localizes to the centrosome specifically during interphase, where it may protect centrosomal CDC2 kinase from inappropriate activation by cytoplasmic CDC25B.

TISSUE SPECIFICITY: Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon.


DOMAIN: SwissProt: O14757 The autoinhibitory region (AIR) inhibits the activity of the kinase domain.

PTM: Phosphorylated by ATR in a RAD17-dependent manner in response to ultraviolet irradiation and inhibition of DNA replication. Phosphorylated by ATM in response to ionizing irradiation. ATM and ATR can both phosphorylate Ser-317 and Ser-345 and this results in enhanced kinase activity. Phosphorylation at Ser-345 also increases binding to 14-3-3 proteins and promotes nuclear retention. Conversely, dephosphorylation at Ser-345 by PPM1D may contribute to exit from checkpoint mediated cell cycle arrest. May also be phosphorylated at Ser-280 by AKT1/PKB, which may promote mono and/or diubiquitination. Also phosphorylated at undefined residues during mitotic arrest, which results in decreased activity. & Ubiquitinated. Mono or diubiquitination promotes nuclear exclusion (By similarity).


SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily. & Contains 1 protein kinase domain.
Product Usage Statements
Quality AssuranceRoutinely evaluated as a substrate for CHK enzymes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -20°C
Packaging Information
Material Size200 µg
Global Trade Item Number
Katalógusszám GTIN
12-414 04053252674747