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SCC252 CH12.LX Mouse Lymphoma Cell Line

CH12.LX mouse lymphoma cell line is ideal for studies of B-cell activation and expansion.

SCC252
≥1X10⁶ cells/vial   
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Áttekintés

Replacement Information
Description
Catalogue NumberSCC252
DescriptionCH12.LX Mouse Lymphoma Cell Line
Alternate Names
  • CH12-LX
  • CH12LX
Background InformationNaïve B-cells differentiate and expand upon antigenic stimulation, undergoing class-switch recombination of immunoglobulin genes and forming antibody-producing plasma cells and memory cells that generate upon recall stimulation (1). B-cell models are useful for elucidating pathways of differentiation and proliferation mechanisms of lymphoma.
The CH12.LX mouse lymphoma cell line is an established and well-characterized B-cell model. CH12.LX cells express Ly-12 and have the characteristic IgM+CD5+CD23-cell surface marker expression of B1a B lymphocytes, as well as expressing CD19, CD11b, MHC II and alpha-4-integrin (3). CH12.LX cells are responsive to a variety of antigens including lipopolysaccharide, expressing the activation protein CD14 (4). CH12.LX cells proliferate rapidly and are ideal for studies of B-cell activation and expansion.

Source:
The CH12.LX cell line was generated from B10.H-2aH4bp/Wts mice immunized with sheep erythrocytes (2).

References:
1. Semin Hematol. 1997; 34(1 Suppl 1): 2-12.
2. Proc Natl Acad Sci USA. 1986; 83(19): 7410-7414.
3. J Immunotoxicol. 2012; 9(2): 129-140.
4. Scand J Immunol. 2000; 51(4): 392-399.
References
Product Information
Applications
ApplicationCH12.LX mouse lymphoma cell line is ideal for studies of B-cell activation and expansion.
Key Applications
  • Cell Culture
Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
Biological Information
Cell Line Type
  • Cancer Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance• Each vial contains ≥ 1X10⁶ viable cells.
• Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of mouse origin and negative for inter-species contamination from rat, human, chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Packaging Information
Material Size≥1X10⁶ cells/vial
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
SCC252 04061841689263