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203118 Biotin-NHS, Water-Soluble

Biotin-NHS, Water-Soluble MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Sulfobiotin-NHS, Sulfosuccinimidobiotin

203118
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Áttekintés

Replacement Information

Products

KatalógusszámCsomagolás Menny./csomag
203118-50MG Muanyagampulla 50 mg
Description
OverviewReacts with primary amines to biotinylate proteins under mild conditions. Since it is water-soluble, this compound can be used to biotinylate cell-surface proteins and proteins that are sensitive to organic solvents.
Catalogue Number203118
Brand Family Calbiochem®
SynonymsSulfobiotin-NHS, Sulfosuccinimidobiotin
References
ReferencesLisanti, M.P., et al. 1989. J. Cell Biol. 109, 2145.
Product Information
FormOff-white solid
Hill FormulaC₁₄H₁₈N₃O₈S₂ · Na
Chemical formulaC₁₄H₁₈N₃O₈S₂ · Na
Quality LevelMQ100
Applications
Biological Information
Purityhomogeneous single spot by TLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Moisture Protect from moisture
Do not freeze Ok to freeze
Special InstructionsUnstable in aqueous solution; reconstitute just prior to use. Following reconstitution in DMSO, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
203118-50MG 04055977221640

Documentation

Biotin-NHS, Water-Soluble MSDS

Title

Safety Data Sheet (SDS) 

Biotin-NHS, Water-Soluble Certificates of Analysis

TitleLot Number
203118

References

Hivatkozások áttekintése
Lisanti, M.P., et al. 1989. J. Cell Biol. 109, 2145.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-May-2008 RFH
SynonymsSulfobiotin-NHS, Sulfosuccinimidobiotin
DescriptionWater-soluble form of biotin-NHS. Water solubility is extremely convenient, eliminating the need to dissolve the reagent in an organic solvent such as dimethylformamide (DMF) and dimethylsulfoxide (DMSO) prior to use.
FormOff-white solid
Recommended reaction conditions
Buffers containing amines (such as Tris or glycine) should be avoided because they can compete with the reaction. It is also very important that the stock solution be prepared just prior to addition of the reaction mixture, because these compounds are prone to hydrolysis by water. Water-soluble crosslinkers can be dissolved in water or buffer at a concentration of 10-25 mM. Phosphate buffers may precipitate sulfo-N-hydroxysuccinimide (sulfo-NHS) containing compounds. In addition, the solid crosslinker can be added directly to the sample to minimize hydrolysis. Hydrolysis is more pronounced in dilute protein solutions, but can be minimized by using more concentrated protein solution (greater than 5 mg/ml). If you prefer to work with stock solutions, we recommend that you dissolve the crosslinker in anhydrous DMSO at a concentration of 10-25 mM. This solution can then be diluted into the buffer of your choice just prior to use. General Handling 1. Store desiccated at 4°C. 2. Warm contents to room temperature completely before opening. Allow at least 30 min. 3. Wipe the vial to remove any moisture. 4. After opening, store under nitrogen. 5. Protect from moisture.
Chemical formulaC₁₄H₁₈N₃O₈S₂ · Na
Purityhomogeneous single spot by TLC
SolubilitydH₂O (4 mg/ml) or DMSO (10 mg/ml)
Storage Protect from moisture
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsUnstable in aqueous solution; reconstitute just prior to use. Following reconstitution in DMSO, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Toxicity Standard Handling
ReferencesLisanti, M.P., et al. 1989. J. Cell Biol. 109, 2145.