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SCT072 BioTracker NIR-ASM NQO1 Live Cell Dye

Live cell imaging fluorescent probe for near-infrared imaging of NQO1 enzyme in cancer cells in vitro and in vivo

SCT072
1 mg  
Purchase on Sigma-Aldrich

Áttekintés

Replacement Information
Description
Catalogue NumberSCT072
DescriptionBioTracker NIR-ASM NQO1 Live Cell Dye
OverviewNAD(P)H quinone oxidoreductase 1 (NQO1) is a cancer-specific target enzyme and accurate detection of NQO1 activity with high sensitivity and selectivity is useful for the early diagnosis of cancer. NQO1 is a two-electron reductase responsible for the detoxification of xenobiotics such as quinones and is abnormally overexpressed in many tumors and intimately linked with multiple carcinogenic processes. compared to healthy tissue, NQO1 expressed at levels of 5−200-fold above in tumors, such as the breast12, lung13,14, prostate15, stomach16, colon17, pancreas18, head, and neck cancer19. Consistent with this, NQO1 has been extensively explored to achieve highly selective and sensitive detection or visualization of tumor-associated events in different cancers.

BioTracker NIR-ASM NQO1 Live Cell Dye demonstrated high selectivity for NQO1 over the other enzymes/substrates such as glutathione, aldehyde dehydrogenase 1, gamma-glutamyl transferase, glutathione peroxidase, apurinic/apyrimidinic endonuclease, cystathionine-β-synthase, cathepsin L, glutathione S-transferase, NADH alone and the combination of nitroreductase and NQO1. Fluorescence only resulted from the specific cleavage of the probe by NQO1 in the presence of its co-factor NADH. NIR-ASM dye was not toxic to both normal and cancer cells even at higher concentrations (up to 100 μM). A high-intensity distribution of signals was obtained for NIR-ASM dye activation in each of the two NQO1-positive cancer cell lines (A549 and H460), while the negative normal cell lines IMR90 and HUVECs produced minimal fluorescence.

Spectral properties:
Excitation: 488 nm
Emission: 644 nm

References:
Punganuru S.R. et al. Characterization of a highly specific NQO1-activated nearinfrared fluorescent probe and its application for in vivo tumor imaging. Sci. Reports. 2019, 9:8577.
Alternate Names
  • Live cell imaging probe
  • NQO1 probe
References
Product Information
Detection methodFluorescence
PresentationLyophilized
Applications
ApplicationLive cell imaging fluorescent probe for near-infrared imaging of NQO1 enzyme in cancer cells in vitro and in vivo
Key Applications
  • Cell Based Assays
Application NotesLive cell fluorescent imaging
Biological Information
Molecular Weight521.6
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssurancePurity: ≥ 94% confirmed by HPLC. Structure confirmed by HNMR, LC-MS and elemental analysis
Molar Mass: 521.6 g/mol
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore BioTracker NIR-ASM NQO1 Live Cell Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Packaging Information
Material Size1 mg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalógusszám GTIN
SCT072 04061841441045